Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Keeping it centered: decoding the activities that regulate yeast histone H3 variant Cse4 and confine it to centromeres.

Frontiers in cell and developmental biology·2026
Same author

Embrace diverse PhD supervision styles - but enforce essential standards.

Nature·2026
Same author

Safeguarding genome integrity: Polo-like kinase Cdc5 and phosphatase Cdc14 orchestrate Topoisomerase II-mediated catenane resolution in mitosis.

Nucleic acids research·2026
Same author

The RioK1 network determines p53 activity at multiple levels.

Cell death discovery·2023
Same author

The Oncogenic Protein Kinase/ATPase RIOK1 Is Up-Regulated via the c-myc/E2F Transcription Factor Axis in Prostate Cancer.

The American journal of pathology·2023
Same author

Rio1 downregulates centromeric RNA levels to promote the timely assembly of structurally fit kinetochores.

Nature communications·2023

Video Experimental Relacionado

Updated: Jul 3, 2026

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

Cdc14B y APC/C abordan el daño en el ADN.

Peter De Wulf1, Rosella Visintin

  • 1Department of Experimental Oncology, European Institute of Oncology, IFOM-IEO Campus, Via Adamello 16, 20139 Milan, Italy.

Cell
|July 30, 2008
PubMed
Resumen

Los reguladores del ciclo celular de la levadura en ciernes Cdc14, APC/C(Cdh1) y Plk1 han sido recableados en células humanas. Esta red ahora controla el ciclo celular en respuesta al daño del ADN.

Área de la Ciencia:

  • Biología celular Biología celular.
  • Biología Molecular Biología Molecular
  • Genética La genética.

Sus antecedentes:

  • La salida mitótica en la levadura en ciernes es un proceso crucial del ciclo celular.
  • Los reguladores clave incluyen Cdc14, APC/C(Cdh1), y Plk1.
  • Comprender estos reguladores es vital para el control del ciclo celular.

Objetivo del estudio:

  • Para investigar el papel de los reguladores de salida mitótica de la levadura en ciernes en las células humanas.
  • Para determinar si la red de proteínas se conserva o se vuelve a cablear en el control del ciclo celular humano.
  • Para explorar la respuesta de esta red al daño del ADN en las células humanas.

Principales métodos:

  • Análisis comparativo de las redes de proteínas en levaduras y células humanas.

Más Videos Relacionados

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

Videos de Experimentos Relacionados

Last Updated: Jul 3, 2026

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

  • Investigando la función de Cdc14, APC/C(Cdh1) y Plk1 en el ciclo celular humano.
  • Evaluación de la progresión del ciclo celular después de la inducción de daño en el ADN.

    • Principales resultados:

    • La red de Cdc14, APC/C(Cdh1) y Plk1 ha sido recableada en las células humanas.
    • Esta red recableada controla la progresión del ciclo celular en respuesta al daño del ADN.
    • Demuestra un mecanismo conservado pero adaptado para la regulación del ciclo celular.

    Conclusiones:

    • La red de control del ciclo celular que involucra a Cdc14, APC/C(Cdh1) y Plk1 es adaptable.
    • Las células humanas utilizan una versión reconfigurada de esta red para la respuesta al daño del ADN.
    • Este hallazgo pone de relieve la plasticidad evolutiva en procesos biológicos fundamentales.