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Videos de Conceptos Relacionados

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

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Video Experimental Relacionado

Updated: Jul 2, 2026

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
08:56

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

Published on: May 5, 2020

Una red de interactomas basada en el dominio de la proteína para la embriogénesis temprana de C. elegans.

Mike Boxem1, Zoltan Maliga, Niels Klitgord

  • 1Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA. mboxem@partners.org

Cell
|August 12, 2008
PubMed
Resumen

Los investigadores mapearon las interacciones de proteínas centrándose en dominios modulares, creando una red de interactomas más completa para el desarrollo de C. elegans. Este enfoque basado en el dominio ofrece nuevos conocimientos sobre la organización y función celular.

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The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging
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The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging

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Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans
07:22

Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans

Published on: May 23, 2020

Videos de Experimentos Relacionados

Last Updated: Jul 2, 2026

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
08:56

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

Published on: May 5, 2020

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging
12:15

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging

Published on: October 3, 2017

Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans
07:22

Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans

Published on: May 23, 2020

Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • Biología de Sistemas Biología de Sistemas.
  • Biología del desarrollo Biología del desarrollo.

Sus antecedentes:

  • Las interacciones proteína-proteína son cruciales para las funciones celulares.
  • Las redes interactómicas existentes a menudo pasan por alto la organización de dominio modular de las proteínas.
  • Comprender las interacciones mediadas por dominio es clave para descifrar redes biológicas complejas.

Objetivo del estudio:

  • Desarrollar una estrategia experimental para identificar los dominios de interacción de las proteínas.
  • Construir una red de interactomas basada en dominios para las proteínas embrionarias tempranas de C. elegans.
  • Para revelar conocimientos sobre la función del centrosoma de C. elegans a través del modelado del interactoma.

Principales métodos:

  • Desarrolló una nueva estrategia experimental para identificar eficientemente los dominios de interacción de las proteínas.
  • Generó una red de interactomas basada en dominios utilizando proteínas de las divisiones celulares embrionarias tempranas de C. elegans.
  • Se identificaron regiones de interacción mínima para más de 200 proteínas.

Principales resultados:

  • Identificó con éxito dominios de interacción y mapeó las interacciones basadas en dominios para más de 200 proteínas.
  • El enfoque desarrollado mejoró la sensibilidad del sistema de dos híbridos, lo que condujo a un interactoma más completo.
  • La red de interactomas basada en el dominio proporcionó nuevos conocimientos sobre la función del centrosoma de C. elegans.

Conclusiones:

  • La modularidad de las proteínas es fundamental para comprender las interacciones proteína-proteína y la organización de la red.
  • La estrategia de modelado de interactomas basada en dominios desarrollada es efectiva y aplicable a varios procesos biológicos.
  • Este enfoque avanza en el estudio de los interactomas y proporciona una base para futuras investigaciones en C. elegans y otros organismos.