Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

S1 guideline sweat gland carcinoma.

Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·2026
Same author

Severe upper gastrointestinal involvement in ulcerative colitis successfully treated with sequential therapy using intravenous tacrolimus and upadacitinib: a case report.

Frontiers in medicine·2026
Same author

The Cancer Immunotherapy Thromboembolism Assessment: A Novel Score for Predicting Thromboembolic Events in Melanoma Patients Treated With Immune Checkpoint Inhibition.

International journal of cancer·2026
Same author

Real-world effectiveness and safety with nivolumab plus ipilimumab or nivolumab alone in patients with or without melanoma brain metastasis: Results from the German noninterventional NICO study.

International journal of cancer·2026
Same author

Feeder-free yet still naïve: improved method for capturing human pluripotent stem cells.

The EMBO journal·2026
Same author

COMBI-EU: Real-World Evidence on Adverse Event Management and Time on Therapy with Adjuvant Dabrafenib Plus Trametinib in Patients with BRAF V600-Mutant Melanoma.

Cancers·2026

Video Experimental Relacionado

Updated: Jun 30, 2026

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
09:43

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Published on: April 23, 2014

Células madre pluripotentes inducidas generadas sin integración viral.

Matthias Stadtfeld1, Masaki Nagaya, Jochen Utikal

  • 1Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, 185 Cambridge Street, Boston, MA 02114, USA.

Science (New York, N.Y.)
|September 27, 2008
PubMed
Resumen
Este resumen es generado por máquina.

Los investigadores crearon células madre pluripotentes inducidas (iPS) de ratón utilizando adenovirus no integrantes, evitando los virus dañinos que integran el genoma. Este método más seguro demuestra que la integración genética no es necesaria para la reprogramación celular exitosa.

Más Videos Relacionados

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids
10:52

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

Published on: June 5, 2015

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
09:45

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Published on: January 1, 2017

Videos de Experimentos Relacionados

Last Updated: Jun 30, 2026

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
09:43

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Published on: April 23, 2014

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids
10:52

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

Published on: June 5, 2015

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
09:45

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Published on: January 1, 2017

Área de la Ciencia:

  • Biología de las células madre Biología de las células madre
  • Biología molecular La biología molecular.
  • Genética La genética.

Sus antecedentes:

  • Las células madre pluripotentes son cruciales para la medicina regenerativa.
  • Los métodos actuales para generar células madre pluripotentes inducidas (iPS) a menudo se basan en la integración de virus, lo que plantea preocupaciones de seguridad.
  • Oct4, Sox2, Klf4 y c-Myc son factores de transcripción clave para la reprogramación celular.

Objetivo del estudio:

  • Desarrollar un método más seguro para generar células madre pluripotentes inducidas (iPS).
  • Para investigar si los vectores virales no integradores pueden reprogramar eficientemente las células somáticas.
  • Para evaluar la pluripotencia y el potencial de desarrollo de las células reprogramadas viralmente.

Principales métodos:

  • Generación de células iPS de ratón a partir de fibroblastos y células hepáticas.
  • Expresión transitoria de Oct4, Sox2, Klf4 y c-Myc utilizando adenovirus no integrados.
  • Análisis de la desmetilación del ADN, la expresión génica de pluripotencia, la formación de teratomas y la contribución de la línea germinal en ratones quiméricos.

Principales resultados:

  • Se generaron con éxito células adenovirales iPS (adeno-iPS) de ratón utilizando una expresión adenoviral transitoria y no integradora.
  • Las células adeno-iPS exhibieron la característica desmetilación del ADN y expresaron marcadores de pluripotencia endógenos.
  • Estas células formaron teratomas y contribuyeron a varios tejidos, incluida la línea germinal, en ratones quiméricos.

Conclusiones:

  • La mutagénesis por inserción no es un requisito previo para la reprogramación in vitro de las células somáticas.
  • La reprogramación adenoviral ofrece una alternativa más segura para generar células iPS.
  • Este método mejorado facilita el estudio de las células madre específicas del paciente y las comparaciones entre las células madre embrionarias y las células iPS.