Video Experimental Relacionado
Updated: Jun 29, 2026
12:02
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Published on: June 6, 2017
Vinculando el ciclo celular a la división asimétrica: Aurora-A fosforila el complejo Par para regular la localización
Frederik Wirtz-Peitz1, Takashi Nishimura, Juergen A Knoblich
1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Dr. Bohr-Gasse 3, 1030 Vienna, Austria.
Cell
|October 16, 2008
Resumen
Un nuevo estudio revela cómo la Aurora-A quinasa desencadena una cascada de fosforilación, lo que lleva a la localización asimétrica de la proteína Numb durante la división celular de Drosophila. Este mecanismo asegura la polaridad celular adecuada y la progresión del ciclo celular.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología del desarrollo Biología del desarrollo.
- Biología Molecular Biología Molecular
Sus antecedentes:
- Las células precursoras neurales de Drosophila exhiben una división asimétrica al segregar la proteína Numb.
- La localización de la proteína Numb cambia de cortical uniforme en la interfase a polarizada en la mitosis.
Objetivo del estudio:
- Para dilucidar el mecanismo molecular que controla la localización Numb asimétrica durante la mitosis.
- Para entender cómo la polaridad celular está vinculada a la progresión del ciclo celular.
Principales métodos:
- Investigó el papel de la Aurora-A quinasa en la localización de la proteína Numb.
- Se analizó la cascada de fosforilación que involucra a Par-6, la proteína quinasa atípica C (aPKC) y las larvas gigantes letales (2) (Lgl).
- Examinó la interacción entre aPKC, Lgl y Bazooka.
Principales resultados:
- La activación de Aurora-A inicia una cascada de fosforilación.
- La aurora-A fosforila el Par-6, activando el aPKC.
- El aPKC activado fosforila Lgl, liberándolo y permitiendo que Bazooka se una, lo que en última instancia conduce a la fosforilación de Numb y la liberación cortical asimétrica.
Conclusiones:
- Se ha identificado un nuevo mecanismo molecular para la localización Numb asimétrica en la mitosis.
- El estudio demuestra el acoplamiento de la polaridad celular a la progresión del ciclo celular a través de una cascada de fosforilación definida.
Más Videos Relacionados
Videos de Conceptos Relacionados
01:27Molecular Factors Affecting Cell Division
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
02:17Separation of Sister Chromatids
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
At the onset of anaphase, separase, a proteolytic enzyme, is...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...