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El gen de desarrollo Viviparous-1 del maíz codifica un nuevo activador transcripcional

D R McCarty¹, T Hattori, C B Carson

¹Vegetable Crops Department, University of Florida, Gainesville 32611.

|September 6, 1991

Resumen

El gen Viviparous-1 (Vp1) del maíz codifica un nuevo factor de transcripción esencial para la maduración de las semillas. Esta proteína activa la expresión génica, lo que sugiere un papel en las respuestas hormonales de las plantas durante el desarrollo de las semillas.

Área de la Ciencia:

Biología Molecular Vegetal Biología Molecular Vegetal
Biología del desarrollo Biología del desarrollo.
Genética La genética.

Sus antecedentes:

El gen Viviparous-1 (Vp1) en el maíz es crucial para regular la maduración de las semillas.
Comprender los mecanismos moleculares de Vp1 es clave para descifrar las vías de desarrollo de las semillas.

Objetivo del estudio:

Caracterizar el producto del gen Vp1 y aclarar su función en el desarrollo de semillas de maíz.
Para identificar los dominios funcionales de la proteína Vp1 responsable de la activación de la transcripción.

Principales métodos:

Clonación del gen Vp1 y caracterización de proteínas.
Fusión de Vp1 con el dominio de unión al ADN GAL4 para identificar secuencias de activación.
Ensayos de genes reporteros en protoplastos de maíz para evaluar el potencial de transactivación.

Principales resultados:

Vp1 codifica una proteína de 73.335 daltones que carece de homología con las proteínas conocidas.
Se identificó un dominio de activación transcripcional ácida dentro de Vp1.1.
Vp1 activó fuertemente la expresión del gen reportero (> 130 veces) en los protoplastos de maíz.
El dominio ácido era esencial para la transactivación y podría ser sustituido funcionalmente por la secuencia del activador VP16.

Conclusiones:

Vp1 funciona como un nuevo factor de transcripción en el maíz.
El dominio ácido identificado es crítico para la actividad de transactivación de Vp1.
Vp1 puede potenciar las respuestas hormonales específicas de la semilla durante el desarrollo.

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