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Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
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Ensamblaje de nanotubos amiloideos dirigidos por nucleobases dirigidos por nucleobases.
Peng Liu1, Rong Ni, Anil K Mehta
1Center for Fundamental and Applied Molecular Evolution, Department of Chemistry, Emory University, Atlanta, Georgia 30322, USA.
Journal of the American Chemical Society
|December 5, 2008
Resumen
Los investigadores crearon nuevos nanotubos mediante la incorporación de nucleobases de citosina en péptidos amiloides. Estas estructuras de autoensamblaje ccAQLVFFA exhiben una arquitectura rica en hojas beta, formando nanotubos bien definidos a través de interacciones de bases complementarias.
Área de la Ciencia:
- Ciencia de los Biomateriales Ciencia de los Biomateriales.
- Química supramolecular de las moléculas.
- Nanotecnología La nanotecnología es la nanotecnología.
Sus antecedentes:
- Los péptidos amiloides son conocidos por su autoensamblaje en estructuras de hoja beta.
- La incorporación de nucleobases en péptidos ofrece nuevas posibilidades funcionales.
- El autoensamblaje controlado es clave para el diseño de nanomateriales avanzados.
Objetivo del estudio:
- Para sintetizar y caracterizar un nuevo nucleobase-peptídeo conjugado (ccAQLVFFA).
- Investigar el comportamiento de autoensamblaje de ccAQLVFFA en estructuras de orden superior.
- Explorar el papel de la complementariedad de la citosina en la dirección de la formación de nanotubos.
Principales métodos:
- Síntesis de péptidos que incorporan nucleobases de citosina.
- Diseminación de rayos X de ángulo pequeño (SAXS) para el análisis estructural.
- Espectroscopia infrarroja con transformación de Fourier (FT-IR) y difracción de rayos X (XRD) para la confirmación del ensamblaje.
- Resonancia Magnética Nuclear (RMN) de estado sólido y dicroísmo lineal para la determinación del registro de hojas beta.
Principales resultados:
- Incorporación exitosa de citosina en el fragmento de péptido amiloide HHQALVFFA, dando como resultado ccAQLVFFA.
- Formación de nanotubos bien definidos (diámetro exterior de 24,8 nm, espesor de pared de 3,3 nm) a un pH de 3 a 4.
- Confirmación del ensamblaje rico en hojas beta con arquitectura beta cruzada.
- Las bases de citosina colocadas perpendicularmente al eje del nanotubo, mejoran el apilamiento de hojas beta a través de interacciones complementarias.
Conclusiones:
- Los péptidos amiloides funcionalizados con citosina pueden autoensamblarse en nanotubos ordenados.
- Las interacciones complementarias de las nucleobases dentro de las cadenas laterales del péptido impulsan la formación de la arquitectura de los nanotubos.
- Este trabajo expande el concepto de codificación de información más allá de los dúplex de ácido nucleico en redes covalentes de autoensamblaje.
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