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El deterioro dependiente de la edad de los complejos de poros nucleares causa una pérdida de la integridad nuclear en
Maximiliano A D'Angelo1, Marcela Raices, Siler H Panowski
1Salk Institute for Biological Studies, Molecular and Cell Biology Laboratory, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
Cell
|January 27, 2009
Resumen
Los complejos de poros nucleares (NPC) son de larga duración en células diferenciadas, a diferencia de las células que se dividen. El envejecimiento conduce al daño de las NPC, aumentando la permeabilidad nuclear y sugiriendo un papel para las NPC en el proceso de envejecimiento.
Área de la Ciencia:
- Biología celular Biología celular.
- Investigación sobre el envejecimiento Investigación sobre el envejecimiento.
- Biología Molecular Biología Molecular
Sus antecedentes:
- Los complejos de poros nucleares (NPC) son esenciales para el transporte nucleocitoplasmático.
- Las NPC se desmontan y vuelven a ensamblar durante la división celular (mitosis).
- El destino y la estabilidad de las NPC en las células no divididas (postmitóticas) siguen siendo en gran medida no caracterizadas.
Objetivo del estudio:
- Para investigar la rotación y la estabilidad de las NPC en células postmitóticas diferenciadas.
- Para determinar si los NPC sufren cambios relacionados con la edad.
- Explorar las consecuencias funcionales de las alteraciones de NPC durante el envejecimiento.
Principales métodos:
- Utilizó técnicas para rastrear la estabilidad de las nucleoporinas (componentes NPC) en células diferenciadas.
- Evalúa la integridad de las NPC y la permeabilidad nuclear en células de diferentes edades.
- Investigó el estado de daño oxidativo de las nucleoporinas en células envejecidas.
Principales resultados:
- Los complejos de poros nucleares (NPC) no presentan rotación en células diferenciadas; las nucleoporinas del andamio son excepcionalmente estables durante toda la vida útil de la célula.
- Un subconjunto de componentes NPC (por ejemplo, Nup153, Nup50) se somete a un intercambio continuo.
- El envejecimiento se correlaciona con el deterioro de las NPC, el aumento de la permeabilidad nuclear y la fuga de proteínas citoplasmáticas al núcleo.
- El daño oxidativo a las nucleoporinas se observa en las células envejecidas.
Conclusiones:
- Los complejos de poros nucleares son estructuras notablemente estables en células diferenciadas.
- Los NPC sufren daños relacionados con la edad, lo que contribuye a un aumento de la permeabilidad nuclear.
- La acumulación de daño en el NPC es un evento significativo en el envejecimiento celular.
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