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La interpretación del gradiente sin alas requiere una autoinhibición inducida por la señalización
Eugenia Piddini1, Jean-Paul Vincent
1MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, UK.
Cell
|January 27, 2009
Resumen
La señalización sin alas en las alas de Drosophila implica una inhibición lateral, donde las células circundantes envían señales inhibidoras. Este proceso no autónomo refina la información posicional y previene el destino de las células ectópicas, lo que sugiere un mecanismo general de interpretación del gradiente morfogénico.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- La señalización celular de las células.
- Genética La genética.
Sus antecedentes:
- Los modelos clásicos de desarrollo asumen que las células interpretan los gradientes de morfogénesis de forma independiente.
- En Drosophila, Wingless (Wg) organiza el eje proximodistal del ala a través de la activación del gen diana dependiente de la dosis.
- Los mecanismos precisos de la interpretación del morfógeno y la formación de patrones aún están bajo investigación.
Objetivo del estudio:
- Investigar las funciones no autónomas de la señalización sin alas en el desarrollo del ala de Drosophila.
- Para dilucidar los mecanismos por los que las células influyen en la respuesta de cada uno a los gradientes de morfogénicos.
- Determinar la contribución de la inhibición lateral a la adquisición de información posicional.
Principales métodos:
- Análisis de los patrones de expresión génica en los discos imaginarios de las alas de Drosophila.
- Manipulación genética para perturbar las vías de señalización de Wingless.
- Observación de los destinos celulares y el patrón de los tejidos.
Principales resultados:
- La señalización sin alas desencadena dos programas inhibidores no autónomos.
- Una señal negativa codificada por 'notum' inhibe la señalización Wg en las células vecinas.
- Las células prospectivas del ala producen una señal no identificada que amortigua la expresión génica diana de Wg.
- La interrupción de la inhibición lateral conduce a destinos celulares ectópicos.
Conclusiones:
- La señalización sin alas emplea al menos dos modos de inhibición lateral para establecer información de posición.
- La inhibición lateral es crucial para la formación precisa de patrones y la prevención de destinos celulares aberrantes.
- La inhibición lateral puede representar un mecanismo general para interpretar los gradientes de morfógenos durante el desarrollo.
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