Video Experimental Relacionado
Updated: May 6, 2026
11:44
A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
Published on: January 24, 2016
11.7K
El programa regulador de supresores de células T coopta el factor de transcripción IRF4 para controlar las respuestas
Ye Zheng1, Ashutosh Chaudhry, Arnold Kas
1Howard Hughes Medical Institute, University of Washington, Seattle, Washington 98195, USA.
Nature
|February 3, 2009
Resumen
Las células T reguladoras (Treg) utilizan el factor de transcripción IRF4 para suprimir las respuestas inmunes T(H) 2. La pérdida de IRF4 en las células Treg perjudica selectivamente la supresión de T(H) 2, lo que lleva a la patología autoinmune.
Área de la Ciencia:
- Inmunología Inmunología.
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
Sus antecedentes:
- Las células T auxiliares (T ((H)) se diferencian en subconjuntos efectores (T ((H) 1, T ((H) 2, T ((H) 17) durante la infección o la autoinmunidad.
- Las células T reguladoras (T(reg)) suprimen las respuestas inmunes, con una diferenciación guiada por el factor de transcripción Foxp3.3.
- La deficiencia de Foxp3 causa enfermedades autoinmunes graves con un aumento de las citoquinas T(H) 1 y T(H) 2.
Objetivo del estudio:
- Investigar el papel del factor de transcripción IRF4 en la función de las células T.
- Determinar si IRF4 es un objetivo directo de Foxp3 y media la supresión de T (reg) de las respuestas T (H) 2.
Principales métodos:
- Análisis de la expresión de IRF4 en células T ((reg) de ratón.
- La ablación condicional del gen Irf4 en las células T.
- Evaluación de las respuestas de las células T(H) 2, la producción de inmunoglobulinas y la patología tisular.
Principales resultados:
- La expresión de Foxp3 se requiere para los altos niveles de IRF4 en las células T de ratón.
- La ablación de Irf4 en las células T (reg) condujo a respuestas desreguladas de T (H) 2 y a la producción de inmunoglobulinas dependientes de IL4.
- La deficiencia de IRF4 específica de la célula resultó en una patología autoinmune distinta caracterizada por la infiltración de células plasmáticas.
Conclusiones:
- Las células T (reg) utilizan IRF4 para suprimir específicamente las respuestas inmunes mediadas por T (H) 2.
- Este mecanismo pone de relieve cómo las células T ((reg) cooptan la maquinaria de diferenciación de células efectoras para controlar respuestas inmunes específicas.
Más Videos Relacionados
08:20In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol
Published on: December 30, 2016
20.1K
08:57Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
Published on: October 6, 2019
9.9K
Videos de Conceptos Relacionados
01:52Eukaryotic Transcription Inhibitors
9.1K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
9.1K
01:31Regulation of the Unfolded Protein Response
2.2K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.2K
01:16TGF - β Signaling Pathway
7.2K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.2K
01:29Somatic to iPS Cell Reprogramming
2.1K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.1K
01:23Regulation of Expression at Multiple Steps
1.4K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.4K
01:21Repressible Operon: trp Operon
2.8K
The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
2.8K