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Videos de Conceptos Relacionados

RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
Protein Folding01:25

Protein Folding

Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Folding01:22

Protein Folding

Overview

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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Estructuras terciarias de ARN similares a las nativas que utilizan un agente de escisión codificado en secuencia y

Costin M Gherghe1, Christopher W Leonard, Feng Ding

  • 1Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599-3290, USA.

Journal of the American Chemical Society
|February 6, 2009
PubMed
Resumen

Este estudio introduce un nuevo método computacional combinado con experimentos de sondeo de la estructura del ARN para modelar con precisión estructuras terciarias complejas de ARN. Este enfoque refina las estructuras de ARN de manera eficiente sin suposiciones previas, ayudando en el estudio de ARN flexibles y funcionalmente importantes.

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Área de la Ciencia:

  • La bioquímica es la bioquímica.
  • Biología computacional Biología computacional.
  • Biología Estructural Biología estructural.

Sus antecedentes:

  • El análisis de las estructuras de ARN de orden superior, particularmente los dominios flexibles y los intermedios plegables, es un desafío.
  • Los métodos existentes luchan con los ARN que requieren flexibilidad conformacional para funcionar.
  • El modelado preciso de la estructura terciaria del ARN es crucial para comprender la función del ARN.

Objetivo del estudio:

  • Desarrollar un enfoque conciso y preciso para modelar la estructura terciaria del ARN.
  • Para superar las limitaciones de los métodos estructurales convencionales para ARN flexibles.
  • Para permitir la determinación de la estructura sin suposiciones previas sobre el plegamiento del ARN.

Principales métodos:

  • Utilizando experimentos fáciles de sondeo de la estructura del ARN, incluida la química SHAPE para la estructura secundaria.
  • Empleando agentes de escisión dirigidos por secuencia para generar información de distancia entre residuos.
  • Interpretación de datos experimentales con un algoritmo de dinámica molecular discreta rápido y de grano grueso.
  • Representación de cada nucleótido de ARN con seudoátomos (fosfato, ribosa, nucleobasa).

Principales resultados:

  • Se han refinado con éxito las posiciones emparejadas de bases en el tRNA de levadura (((Asp) a 4 Å de desviación de la raíz media cuadrada (rmsd).
  • Se logró un refinamiento estructural de alta resolución sin información estructural preexistente.
  • Demostró un algoritmo computacional optimizado para la resolución y la velocidad sin la intervención del usuario.

Conclusiones:

  • Este enfoque experimental y computacional combinado ofrece una nueva y poderosa herramienta para el modelado de la estructura del ARN.
  • El método tiene el potencial de producir modelos similares a los nativos para diversos ARN funcionalmente importantes.
  • Aborda la necesidad de nuevos enfoques para estudiar los ARN intratables por las técnicas convencionales de biología estructural.