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Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
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La escisión de la membrana por el complejo ESCRT-III.

Thomas Wollert1, Christian Wunder, Jennifer Lippincott-Schwartz

  • 1Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, US Department of Health and Human Services, Bethesda, Maryland 20892, USA.

Nature
|February 24, 2009
PubMed
Resumen
Este resumen es generado por máquina.

El complejo de clasificación endosómica requerido para el transporte (ESCRT) -III de las proteínas Vps20, Snf7 y Vps24 puede desprender las vesículas. La actividad de la ATPasa Vps4 es crucial para el reciclaje de ESCRT-III y los ciclos múltiples de brotación.

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Área de la Ciencia:

  • Biología celular Biología celular.
  • Biología Molecular Biología Molecular
  • La bioquímica es la bioquímica.

Sus antecedentes:

  • El complejo de clasificación endosómica requerido para la maquinaria de transporte (ESCRT) es vital para los procesos celulares como la formación de cuerpos multivesiculares, el brote viral y la citocinesis.
  • ESCRT-III, un componente central, comprende proteínas que se ensamblan en filamentos para impulsar eventos de remodelación de la membrana.

Objetivo del estudio:

  • Reconstituir y visualizar el brote y la escisión de las vesículas intralumenal mediadas por ESCRT-III.
  • Determinar los requisitos mínimos de proteínas para la función ESCRT-III en el desprendimiento y el reciclaje de las vesículas.

Principales métodos:

  • Reconstitución del brote y la escisión de las vesículas intralumenares dependientes de la ESCRT-III utilizando vesículas unilamellares gigantes.
  • Visualización del proceso utilizando microscopía de fluorescencia.

Principales resultados:

  • Tres subunidades ESCRT-III (Vps20, Snf7, Vps24) fueron suficientes para el desprendimiento de la vesícula intralumenal.
  • Vps2 y la actividad ATPasa de Vps4 fueron esenciales para el reciclaje de ESCRT-III y múltiples rondas de brotación de vesículas.
  • El complejo ESCRT-III y Vps4 funcional mínimo representa un antiguo conjunto conservado de proteínas.

Conclusiones:

  • El estudio identifica los componentes mínimos de ESCRT-III necesarios para la escisión de las vesículas.
  • La actividad de la ATPasa mediada por Vps4 es crítica para el ciclo dinámico de ESCRT-III, permitiendo la remodelación sostenida de la membrana.
  • Estos hallazgos arrojan luz sobre la conservación evolutiva del sistema ESCRT.