Video Experimental Relacionado
Updated: May 6, 2026
05:54
Microbead Implantation in the Zebrafish Embryo
Published on: July 30, 2015
12.5K
La señalización del FGF durante el desarrollo embrionario regula la longitud de los cilios en diversos epitélios
Judith M Neugebauer1, Jeffrey D Amack, Annita G Peterson
1Department of Neurobiology and Anatomy, University of Utah School of Medicine, Eccles Institute of Human Genetics, Building 533, Room 3160, 15 North 2030 East, Salt Lake City, Utah 84112-5330, USA.
Nature
|February 27, 2009
Resumen
La señalización del factor de crecimiento de los fibroblastos (FGF) es crucial para regular la longitud y la función de los cilios en el desarrollo embrionario de los vertebrados. Esta vía controla la longitud de los cilios al influir en los genes de transporte intraflagellares, impactando en varios tejidos.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología del desarrollo Biología del desarrollo.
- Biología Molecular Biología Molecular
Sus antecedentes:
- Los cilios son órganos vitales de la superficie celular involucrados en el desarrollo embrionario y la señalización celular.
- Las vías de señalización célula-célula que gobiernan la longitud de los cilios siguen siendo en gran medida desconocidas.
Objetivo del estudio:
- Investigar el papel de la señalización del factor de crecimiento de los fibroblastos (FGF) en la regulación de la longitud y función de los cilios.
- Para dilucidar los mecanismos moleculares por los cuales la señalización FGF controla la longitud de los cilios.
Principales métodos:
- Morpholino knockdown del receptor FGF 1 (Fgfr1) en peces cebra y embriones de Xenopus.
- La expresión del receptor dominante negativo del FGF (DN-Fgfr1).
- Inhibición farmacológica de la señalización del FGF utilizando SU5402.2.
- Análisis de la longitud de los cilios, el flujo de fluidos y la expresión génica (foxj1, rfx2, ift88).
Principales resultados:
- La inhibición de la señalización de FGF (Fgfr1 knockdown, DN-Fgfr1, SU5402) redujo consistentemente la longitud de los cilios en diversos tejidos (vesícula de Kupffer, vesícula óptica, conductos pronefáricos, placa de techo gastrocoel).
- La reducción de la longitud de los cilios en la vesícula de Kupffer del pez cebra perturba el flujo direccional del fluido esencial para la formación del eje izquierda-derecha.
- La señalización de FGF regula la longitud de los cilios a través de la vía de transporte intraflagellar Fgf8/Fgf24-Fgfr1, regulando a la baja la expresión de foxj1, rfx2 e ift88.
Conclusiones:
- La señalización del FGF juega un papel fundamental y conservado en la regulación de la longitud de los cilios en múltiples tejidos de vertebrados.
- La desregulación de la señalización del FGF puede contribuir a defectos del desarrollo y enfermedades a través de la función deteriorada de los cilios.
- Los hallazgos revelan una vía conservada mediada por FGF que controla la longitud de los cilios a través del transporte intraflagelar.
Más Videos Relacionados
Videos de Conceptos Relacionados
01:56Gastrulation
53.1K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
53.1K
01:51Determination
16.4K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
16.4K
03:14Notch Signaling Pathway
4.6K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.6K
01:22Microtubules in Signaling
1.5K
The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
1.5K
01:39Mechanism of Filopodia Formation
2.5K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.5K
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
1.8K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
1.8K