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Una subunidad reguladora PP2A regula la señalización de la insulina/IGF-1 de C. elegans mediante la modulación de la
Srivatsan Padmanabhan1, Arnab Mukhopadhyay, Sri Devi Narasimhan
1University of Massachusetts Medical School, Worcester, 01605, USA.
Cell
|March 3, 2009
Resumen
El estudio identifica PPTR-1, una subunidad de B56, como un regulador clave de la vía de señalización de insulina / IGF-1 (IIS) en gusanos. PPTR-1 desfosforila AKT, influyendo en la esperanza de vida y el metabolismo, con funciones conservadas en los mamíferos.
Área de la Ciencia:
- Las vías de señalización celular.
- Biología molecular La biología molecular.
- Genética La genética.
Sus antecedentes:
- La cascada de señalización de insulina / IGF-1 (IIS) es crucial para regular la esperanza de vida, el metabolismo y la respuesta al estrés.
- El IIS es controlado principalmente por la fosforilación de sus componentes por las proteínacinasas específicas de la serina/treonina.
Objetivo del estudio:
- Para identificar las fosfatasas de proteínas serina/treonina que contrarrestan la actividad de la quinasa en la vía IIS.
- Investigar el papel de la fosfatasa identificada, PPTR-1, en la regulación de los fenotipos asociados al IIS.
Principales métodos:
- Pantalla de interferencia de ARN (RNAi) para identificar las fosfatasas.
- Análisis de los fenotipos asociados a la vía IIS (duración de la vida, formación de la duración, resistencia al estrés, almacenamiento de grasa).
- Análisis de fosforilación de AKT-1 y Akt. de mamíferos.
Principales resultados:
- PPTR-1, una subunidad reguladora B56 de PP2A, fue identificada como un regulador clave del IIS.
- La modulación de PPTR-1 tiene un impacto en la esperanza de vida, la duración, la resistencia al estrés y el almacenamiento de grasa en C. elegans.
- PPTR-1 desfosforila AKT-1 en Thr 350, conservado en la regulación B56beta de los mamíferos de Akt en Thr 308.
- Esta regulación afecta a la localización y la actividad del DAF-16.
Conclusiones:
- La subunidad reguladora B56 juega un papel conservado en la regulación de la señalización de la insulina a través de la desfosforilación de AKT.
- Los hallazgos tienen implicaciones significativas para la comprensión y el tratamiento potencial de enfermedades como el cáncer y la diabetes.
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