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In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
Published on: July 25, 2019
Origen y función de las proteínas similares a la ubiquitina
1Yale University, Department of Molecular Biophysics & Biochemistry, 266 Whitney Avenue, PO Box 208114, New Haven, Connecticut 06520, USA. mark.hochstrasser@yale.edu
Nature
|March 28, 2009
Resumen
Las proteínas similares a la ubiquitina (UBL) modifican las proteínas eucariotas, controlando los procesos celulares vitales. La evidencia sugiere que la conjugación UBL evolucionó antes que los eucariotas, originándose en sistemas procariotas.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
- Biología evolutiva Biología evolutiva.
Sus antecedentes:
- Las proteínas eucariotas sufren modificaciones como la conjugación de la ubiquitina y la proteína similar a la ubiquitina (UBL).
- Las UBL regulan procesos fisiológicos cruciales, incluidas las interacciones de las proteínas con el proteosoma y el reclutamiento de la cromatina.
- Estas modificaciones son a menudo transitorias y mediadas por sistemas enzimáticos específicos.
Objetivo del estudio:
- Para explorar los orígenes evolutivos de los sistemas de conjugación UBL-proteína.
- Para investigar la posible ascendencia procariota de las vías de modificación de UBL.
- Para determinar si la conjugación UBL es anterior a la aparición de los eucariotas.
Principales métodos:
- Análisis comparativo de los sistemas de modificación de proteínas.
- Examen de las familias de enzimas involucradas en la conjugación y la desconjugación de UBL.
- Análisis bioinformático de secuencias de proteínas y relaciones evolutivas.
Principales resultados:
- La modificación de la proteína UBL es un mecanismo regulador muy extendido en los eucariotas.
- La evidencia indica un vínculo entre los sistemas UBL y los sistemas de transferencia de azufre de los procariotas.
- Las proteínas homólogas a las enzimas UBL-conjugantes y deconjugantes estaban presentes en el último antepasado común de los eucariotas.
Conclusiones:
- La conjugación UBL-proteína probablemente evolucionó de los sistemas procariotas.
- El mecanismo fundamental para la conjugación UBL es anterior a la evolución eucariota.
- La modificación UBL es un antiguo proceso biológico con profundas raíces evolutivas.
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