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Caracterización biomolecular y secuencias de proteínas del hadrosaurio campaniano B. canadensis
Mary H Schweitzer1, Wenxia Zheng, Chris L Organ
1North Carolina State University, Raleigh, NC 27695, USA. schweitzer@ncsu.edu
Science (New York, N.Y.)
|May 2, 2009
Resumen
Las proteínas endógenas, incluido el colágeno, se conservaron en un hadrosaurio de 80 millones de años, Brachylophosaurus canadensis. Este hallazgo apoya el vínculo evolutivo pájaro-dinosaurio y confirma que la preservación molecular en los dinosaurios no es rara.
Área de la Ciencia:
- Paleontología Paleontología.
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
Sus antecedentes:
- La preservación del material molecular endógeno en los dinosaurios no aviares es un tema de debate científico en curso.
- Los hallazgos anteriores sugirieron que la preservación molecular podría ser rara, con pruebas limitadas de especímenes específicos.
Objetivo del estudio:
- Para investigar la presencia y la naturaleza de las proteínas endógenas conservadas en un espécimen de hadrosaurio campaniano.
- Para proporcionar más evidencia a favor o en contra de la preservación molecular generalizada en los dinosaurios del Cretácico.
- Utilizar análisis filogenéticos para confirmar el origen endógeno de los péptidos de colágeno conservados.
Principales métodos:
- Análisis microestructural de fragmentos óseos y tejidos blandos.
- Pruebas inmunológicas para detectar proteínas específicas.
- Espectrometría de masas para secuenciar péptidos de colágeno.
- Análisis filogenético para determinar las relaciones evolutivas.
Principales resultados:
- Múltiples líneas de evidencia indican la preservación de material proteico endógeno, incluidas las proteínas de la matriz ósea y de los vasos, en Brachylophosaurus canadensis.
- Los análisis filogenéticos del colágeno secuenciado de Brachylophosaurus apoyan firmemente el clado ave-dinosaurio.
- Los hallazgos son consistentes con un origen endógeno para los péptidos de colágeno conservados.
- Estos resultados corroboran los hallazgos anteriores de Tyrannosaurus rex, lo que indica que la preservación molecular no es única.
Conclusiones:
- El material endógeno proteico se conserva de manera demostrable en un hadrosaurio de 80 millones de años.
- La preservación del colágeno apoya el vínculo evolutivo entre las aves y los dinosaurios.
- La preservación molecular en los dinosaurios del Cretácico se confirma como un fenómeno recurrente, no un evento aislado.
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