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13:51
Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics
Published on: February 18, 2009
La metatranscriptómica revela pequeños ARN microbianos únicos en la columna de agua del océano
Yanmei Shi1, Gene W Tyson, Edward F DeLong
1Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Nature
|May 16, 2009
Resumen
Los estudios metatranscriptómicos revelan que muchas secuencias de ARN microbiano son ARN pequeños (ARNm), incluidos los nuevos. Estos sRNA juegan un papel en la adaptación microbiana y en procesos ambientalmente relevantes como la adquisición de nutrientes.
Área de la Ciencia:
- Microbiología Microbiología.
- La genómica es la genómica.
- La bioinformática es la bioinformática.
Sus antecedentes:
- Los estudios metatranscriptómicos analizan la expresión génica microbiana directamente a partir de muestras ambientales.
- Análisis anteriores identificaron muchas secuencias de ARN desconocidas, lo que sugiere proteínas no caracterizadas.
Objetivo del estudio:
- Para investigar la naturaleza de las secuencias de ADN complementarias previamente no caracterizadas que se encuentran en los datos metatranscriptómicos microbianos.
- Identificar y caracterizar los nuevos ARN pequeños (ARNm) y sus funciones potenciales en las comunidades microbianas naturales.
Principales métodos:
- Análisis de secuencias de ADN complementarias de la pirosecuenciación del ARN total de ensamblajes microbianos naturales.
- Mapeo de sRNAs putativos (psRNAs) a las regiones intergénicas de los genomas microbianos.
- Evaluación de las estructuras secundarias y los genes de acompañamiento para los indicadores de la función reguladora.
- Examen de la variación dependiente de la profundidad y mapeo específico del genoma de los psRNAs.
Principales resultados:
- Una fracción significativa de las secuencias de ADNc en los conjuntos de datos metatranscriptómicos son conocidos y nuevos ARN pequeños (ARNs).
- Los nuevos psRNA se asignan a regiones intergénicas, poseen estructuras secundarias conservadas y están cerca de genes reguladores potenciales.
- Las variaciones de psRNA se correlacionan con la distribución taxonómica y sugieren roles en la adaptación de nicho.
- El análisis de Pelagibacter reveló elementos reguladores conocidos y nuevos dentro de los ARNPs ps.
Conclusiones:
- La metatranscriptómica es una herramienta poderosa para descubrir la diversidad, distribución y abundancia de ARNs en comunidades microbianas.
- Los sRNA identificados están involucrados en procesos ambientales críticos, incluido el metabolismo del carbono y la adquisición de nutrientes.
- Este estudio amplía nuestra comprensión de las redes reguladoras microbianas y las estrategias de adaptación.
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