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Updated: Jun 22, 2026

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
10:52

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Published on: January 6, 2016

La transciclación del fosfatidilinositol catalizado por la fosfolipasa C de Streptomyces antibioticus fue catalizada

Chuan Bai1, Li Zhao, Mario Rebecchi

  • 1Department of Medicinal Chemistry and Pharmacognosy, The University of Illinois at Chicago, Chicago, Illinois 60612, USA.

Journal of the American Chemical Society
|June 5, 2009
PubMed
Resumen

La fosfolipasa C (saPLC1) específica para el fosfatidilinositol dependiente de calcio de Streptomyces antibioticus divide el fosfatidilinositol a través de un inusual mecanismo de 1,6-ciclización. Esto genera inositol trans-1,6-fosfato cíclico, confirmado mediante la comparación de productos enzimáticos con estándares sintetizados quimioenzimáticamente.

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