La exocitosis de las vesículas post-Golgi está regulada por componentes de la maquinaria endocítica
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Updated: Jun 22, 2026
04:50
Imaging FITC-dextran as a Reporter for Regulated Exocytosis
Published on: June 20, 2018
Jyoti K Jaiswal1, Victor M Rivera, Sanford M Simon
1The Rockefeller University, 1230 York Avenue, Box 304, New York, NY 10065, USA. jaiswaj@rockefeller.edu
Cell
|July 1, 2009
Resumen
Las vesículas post-Golgi usan principalmente la exocitosis de beso y huida, no la fusión desencadenada por el calcio, para entregar carga a la superficie celular. Este proceso, regulado por la maquinaria endocítica, permite la liberación parcial o completa del contenido de la vesícula.
Área de la Ciencia:
- Biología celular Biología celular.
- El tráfico de membranas.
- La exocitosis es la exocitosis.
Sus antecedentes:
- Las vesículas post-Golgi transportan cargas biosintéticas a la superficie celular.
- Los mecanismos moleculares que rigen la fusión de estas vesículas siguen siendo en gran medida desconocidos.
Objetivo del estudio:
- Para investigar los mecanismos de la fusión de las vesículas post-Golgi y la liberación de la carga.
- Determinar el modo predominante de exocitosis para estas vesículas.
Principales métodos:
- Desarrolló un sistema para monitorear simultáneamente la liberación de carga luminal y de membrana de las vesículas individuales.
- Se observaron eventos de fusión de vesículas y se analizaron los patrones de liberación de carga.
Principales resultados:
- La exocitosis de la vesícula post-Golgi no es desencadenada por el calcio.
- La exocitosis de beso y huida, caracterizada por una liberación parcial o nula de la carga de la membrana, es el modo predominante.
- El cierre prematuro de los poros de fusión, modulado por clatrina, actina y dinamina, causa la liberación parcial de la carga.
Conclusiones:
- La exocitosis de beso y huida es el mecanismo principal para la fusión de vesículas post-Golgi.
- Los componentes de la maquinaria endocítica regulan la extensión de la entrega de carga durante la exocitosis.
- Estos hallazgos aclaran nuevos mecanismos en el transporte y entrega de carga biosintética.
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