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The Replisome

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...

The Replisome

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...

Translesion DNA Polymerases

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
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Overview of Transposition and Recombination

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Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...

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DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
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Updated: Jun 20, 2026

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

Published on: February 25, 2017

La transposición al ADN replicante ocurre a través de la interacción con el factor de procesividad.

Adam R Parks¹, Zaoping Li, Qiaojuan Shi

¹Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.

|August 26, 2009

Resumen

Este resumen es generado por máquina.

El transposón bacteriano Tn7 utiliza la proteína TnsE para atacar el ADN en replicación. TnsE interactúa con la maquinaria de replicación del ADN, guiando la transposición a los sitios de replicación activos.

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Área de la Ciencia:

Biología Molecular Biología Molecular
Genética La genética.
Microbiología Microbiología.

Sus antecedentes:

El transposón bacteriano Tn7 exhibe una preferencia por insertarse en el ADN que se replica activamente.
Esta transposición está mediada por la proteína TnsE codificada por el transposón.

Objetivo del estudio:

Para investigar la interacción entre TnsE y la maquinaria de replicación del ADN del huésped.
Para dilucidar el mecanismo por el cual Tn7 se dirige activamente a la replicación del ADN.

Principales métodos:

Ensayos bioquímicos in vivo e in vitro para el estudio de las interacciones proteína-proteína.
Reconstitución de la reacción de transposición TnsABC+E utilizando proteínas y ADN purificados.
Análisis de la selección del sitio de transposición en relación con la replicación del ADN.

Principales resultados:

TnsE interactúa física y funcionalmente con el factor de procesividad de la replicación del ADN.
Se reconstituyó con éxito un sistema de transposición in vitro (TnsABC+E).
Se demostró que el factor de procesividad reordena los eventos de transposición mediados por TnsE, reflejando el sesgo de replicación in vivo.

Conclusiones:

La interacción de TnsE con la maquinaria de replicación es clave para la selección del sitio objetivo de Tn7.
Este mecanismo permite que Tn7 se transponga preferentemente al ADN que se replica activamente.
Mecanismos similares pueden ser empleados por otros elementos genéticos móviles.