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Los factores de transcripción SPL regulados por miR156 definen una vía de floración endógena en la Arabidopsis

Jia-Wei Wang¹, Benjamin Czech, Detlef Weigel

¹Department of Molecular Biology, Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany.

|August 26, 2009

Resumen

El tiempo de floración en las plantas está controlado por dos vías: el complejo FT / FD y los genes SPL dirigidos a microRNA156. Estas vías convergen para promover el desarrollo reproductivo mediante la activación de genes de caja MADS.

Área de la Ciencia:

Biología Vegetal Biología Vegetal
Genética molecular La genética molecular.
Biología del desarrollo Biología del desarrollo.

Sus antecedentes:

El tiempo de floración es un proceso de desarrollo crucial influenciado por señales ambientales como la longitud del día.
La proteína FLOWERING LOCUS T (FT) y el factor de transcripción FLOWERING TIME BZIP (FD) forman un complejo que desencadena la transición a la floración.
Los microARN desempeñan funciones reguladoras en el desarrollo de las plantas, pero su participación específica en las vías de floración es compleja.

Objetivo del estudio:

Para investigar el papel de los genes SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) dirigidos a microRNA156 en el control de la floración.
Para determinar la relación entre la vía FT/FD y la vía miR156/SPL en la regulación del tiempo de floración.
Aclarar los mecanismos moleculares por los cuales estas vías convergen para promover el desarrollo reproductivo.

Principales métodos:

Análisis de los patrones de expresión génica relacionados con el tiempo de floración.
Investigando las interacciones regulatorias entre los genes FT/FD, miR156 y SPL.
Identificación de objetivos aguas abajo de FT / FD y SPL en el ápice de disparo.

Principales resultados:

Los genes SPL funcionan tanto aguas abajo de FT / FD y como parte de una vía de floración endógena separada.
Una disminución en los niveles de miR156 conduce a un aumento en los niveles de SPL, creando un entorno permisivo para la floración.
FT / FD y SPLs convergen en objetivos superpuestos, con SPLs que activan directamente los genes de la caja MADS.

Conclusiones:

El módulo miR156/SPL actúa independientemente, pero converge con el módulo FT/FD para controlar el tiempo de floración.
Ambas vías, redundante y de avance de alimentación, regulan la activación de los genes de la caja MADS que promueven la floración.
Este estudio revela un marco molecular sofisticado que integra señales ambientales y endógenas para el control de la floración.

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