Video Experimental Relacionado
Updated: Jun 20, 2026
07:23
Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
Published on: June 15, 2016
Definición de los mecanismos que regulan la transcripción de la ARN polimerasa II in vivo
Nicholas J Fuda1, M Behfar Ardehali, John T Lis
1Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA.
Nature
|September 11, 2009
Resumen
El análisis de la distribución de la ARN polimerasa II revela los pasos clave de la regulación génica. Esto ayuda a entender los ciclos de transcripción e identificar los factores reguladores en los eucariotas.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Genética La genética.
- La bioquímica es la bioquímica.
Sus antecedentes:
- Los genomas eucariotas contienen miles de genes que codifican el ARN mensajero.
- La transcripción génica es un proceso fundamental regulado por una compleja maquinaria molecular.
- La ARN polimerasa II es la enzima principal responsable de la transcripción de los genes que codifican las proteínas.
Objetivo del estudio:
- Para investigar la distribución y el estado de la ARN polimerasa II en todo el genoma eucariota.
- Para obtener información sobre los mecanismos de la transcripción y su regulación.
- Identificar los pasos que limitan la velocidad y los factores reguladores clave en el ciclo de transcripción.
Principales métodos:
- Mapeo de todo el genoma de la distribución de la ARN polimerasa II.
- Análisis de la acumulación de ARN polimerasa II en ubicaciones genómicas específicas.
- Integración de los datos de mapeo de los factores de transcripción.
Principales resultados:
- Puntos específicos identificados en el ciclo de transcripción donde se acumula la ARN polimerasa II.
- Etapas limitantes de velocidad caracterizadas que afectan la progresión de la transcripción.
- Distribución correlacionada de la ARN polimerasa II con sitios de unión al factor de transcripción.
Conclusiones:
- Comprender la dinámica de la ARN polimerasa II proporciona información crucial sobre la regulación de los genes.
- Estos enfoques revelan generalidades mecanicistas y diversidades en la regulación génica eucariota.
- El mapeo de la ARN polimerasa II y los factores de transcripción es clave para descifrar las redes reguladoras.
Más Videos Relacionados
09:21Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
Published on: October 22, 2018
10:59Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
Published on: May 13, 2019
Videos de Conceptos Relacionados
02:36RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
02:36RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
01:23Regulation of Expression at Multiple Steps
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
01:23Transcriptional Regulation: Riboswitches
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...