Search research articles

ACERCA DE JoVE

Visión General Liderazgo Blog Centro de Ayuda JoVE

AUTORES

Proceso de Publicación Consejo Editorial Alcance y Políticas Revisión por Pares Preguntas Frecuentes Enviar

BIBLIOTECARIOS

Testimonios Suscripciones Acceso Recursos Consejo Asesor de Bibliotecas Preguntas Frecuentes

INVESTIGACIÓN

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archivo

EDUCACIÓN

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Centro de Recursos para Profesores Sitio de Profesores

Términos y Condiciones de Uso

Política de Privacidad

Videos de Conceptos Relacionados

Chromatin Structure and RNA Splicing

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

Chromatin Structure Regulates pre-mRNA Processing

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

Chromosome Structure

Chromosome Structure

A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

Cis-regulatory Sequences

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...

Cis-regulatory Sequences

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por

Same author

The Topological Regulatory Logic of noncoding RNA-mediated gene expression.

Genome research·2026

Same author

Author Correction: MaizeCODE reveals bi-directionally expressed enhancers that harbor molecular signatures of maize domestication.

Nature communications·2025

Same author

MaizeCODE reveals bi-directionally expressed enhancers that harbor molecular signatures of maize domestication.

Nature communications·2024

Same author

The EN-TEx resource of multi-tissue personal epigenomes & variant-impact models.

Cell·2023

Same author

Long non-coding RNAs: definitions, functions, challenges and recommendations.

Nature reviews. Molecular cell biology·2023

Same author

Author Correction: Expanded encyclopaedias of DNA elements in the human and mouse genomes.

Nature·2022

Same journal

Incoming US science academy chief vows to 'double down' on research.

Nature·2026

Same journal

Author Correction: Synthesis of enantioenriched atropisomers by biocatalytic deracemization.

Nature·2026

Same journal

Electrodeposited self-assembled molecules for perovskite photovoltaics.

Nature·2026

Same journal

Neutrino's nursery found: the 'Shadow Blaster'.

Nature·2026

Same journal

Dementia risk in middle-aged people linked to a blood protein.

Nature·2026

Same journal

Daily briefing: What's really happening with trust in science.

Nature·2026

Ver todos los artículos relacionados

Search research articles

Video Experimental Relacionado

Updated: Jun 20, 2026

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

Published on: April 30, 2011

Implicaciones de las transcripciones quiméricas no co-lineales.

Thomas R Gingeras¹

¹Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA. gingeras@cshl.edu

|September 11, 2009

Resumen

Este resumen es generado por máquina.

Los investigadores descubrieron transcripciones no co-lineales, conocidas como ARN quiméricos, en organismos desde gusanos hasta humanos. Estas transcripciones, formadas por eventos genéticos o post-transcripcionales, desafían nuestra comprensión del contenido de información del genoma.

Más Videos Relacionados

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

Published on: November 1, 2014

Computational Analysis Tutorial for Chimeric Small Noncoding RNA: Target RNA Sequencing Libraries

Computational Analysis Tutorial for Chimeric Small Noncoding RNA: Target RNA Sequencing Libraries

Published on: December 1, 2023

Videos de Experimentos Relacionados

Last Updated: Jun 20, 2026

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

Published on: April 30, 2011

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

Published on: November 1, 2014

Computational Analysis Tutorial for Chimeric Small Noncoding RNA: Target RNA Sequencing Libraries

Computational Analysis Tutorial for Chimeric Small Noncoding RNA: Target RNA Sequencing Libraries

Published on: December 1, 2023

Área de la Ciencia:

La genómica es la genómica.
Biología Molecular Biología Molecular
ARN Biología Biología ARN

Sus antecedentes:

La secuenciación profunda revela transcriptomas, el conjunto completo de transcripciones de ARN.
Algunas transcripciones exhiben estructuras no co-lineales, con segmentos de regiones distantes del ADN.

Objetivo del estudio:

Investigar el origen y las implicaciones de las transcripciones no co-lineales.
Comprender los mecanismos detrás de la formación de ARN quimérico en los eucariotas superiores.

Principales métodos:

Secuenciación profunda de transcriptomas en diversas especies.
Análisis de la composición de la secuencia de ARN y el origen genómico.

Principales resultados:

Transcripciones quiméricas identificadas compuestas por segmentos de ADN no contiguos.
ARN quiméricos observados que surgen tanto de reordenamientos genéticos como de eventos post-transcripcionales.
Destacó el entendimiento limitado de la transespliación en eucariotas superiores.

Conclusiones:

Los ARN quiméricos son una característica significativa en todo el reino animal.
Estos hallazgos amplían la complejidad conocida de la información del genoma y su disposición.
Se necesita más investigación para dilucidar la formación de ARN quimérico en eucariotas superiores.