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RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

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Video Experimental Relacionado

Updated: Jun 20, 2026

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

RNAi en las levaduras en ciernes.

Ines A Drinnenberg1,2, David E Weinberg1,2,3, Kathleen T Xie1,2,3

  • 1Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.

Science (New York, N.Y.)
|September 12, 2009
PubMed
Resumen
Este resumen es generado por máquina.

La interferencia de ARN (RNAi) está presente en las levaduras en ciernes como Saccharomyces castellii, utilizando nuevas proteínas Dicer. La reconstitución de RNAi en Saccharomyces cerevisiae silencia las retrotransposones, ofreciendo nuevas herramientas de investigación.

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Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

Published on: February 27, 2026

Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • Genética La genética.
  • Biología de la levadura Biología de la levadura.

Sus antecedentes:

  • La interferencia de ARN (RNAi) es un mecanismo de silenciamiento de genes conservado en los eucariotas.
  • La vía RNAi ha estado notablemente ausente en el organismo modelo Saccharomyces cerevisiae.
  • Investigaciones anteriores indicaron una pérdida de ARNi en ciertas especies de levaduras en ciernes.

Objetivo del estudio:

  • Para investigar la presencia y el mecanismo de RNAi en especies de levadura en ciernes más allá de Saccharomyces cerevisiae.
  • Para identificar las proteínas específicas involucradas en el RNAi en estas especies.
  • Explorar el potencial de la reconstitución de ARNi en Saccharomyces cerevisiae para el estudio del silenciamiento de genes.

Principales métodos:

  • Genómica comparativa y análisis molecular de los componentes de la vía de RNAi en Saccharomyces castellii y Candida albicans.
  • Identificación y caracterización de las proteínas Dicer no canónicas.
  • Análisis funcionales para evaluar la actividad del ARNi y el silenciamiento del gen objetivo.
  • Manipulación genética de Saccharomyces cerevisiae para introducir y probar los componentes de ARNi de S. castellii.

Principales resultados:

  • Se detectó RNAi en Saccharomyces castellii y Candida albicans, utilizando proteínas Dicer no canónicas.
  • Los pequeños ARN interferentes generados en estas especies se dirigen principalmente a los elementos transponibles y a las repeticiones subteloméricas Y'.
  • Los mutantes con deficiencia de ARNi en S. castellii exhibieron niveles elevados de ARN mensajero Y'.
  • La introducción de S. castellii Dicer y Argonaute restauró el RNAi en Saccharomyces cerevisiae, lo que condujo al silenciamiento de los retrotransposones endógenos.

Conclusiones:

  • Se ha identificado una nueva clase de proteínas Dicer funcionales en el RNAi en levaduras en ciernes.
  • El estudio reintroduce la herramienta RNAi para investigar el silenciamiento genético en levaduras en ciernes.
  • Esta investigación permite la aplicación de sistemas de levadura en ciernes para estudiar los mecanismos de RNAi.