Video Experimental Relacionado
Updated: Aug 14, 2026
07:13
Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer
Published on: March 26, 2014
Evidencia que implica al menos dos genes en el cromosoma 17p en la carcinogénesis del seno
C Coles1, A M Thompson, P A Elder
1Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh, UK.
Lancet (London, England)
|September 29, 1990
Resumen
Los investigadores mapearon la pérdida de heterocigosidad en el cromosoma 17 en pacientes con cáncer de mama. Un gen regulador cerca del gen p53 en 17p13-3 está implicado en la mayoría de los cánceres de mama.
Área de la Ciencia:
- Genética La genética.
- Oncología Oncología.
- Biología Molecular Biología Molecular
Sus antecedentes:
- La pérdida de heterocigosidad (LOH) en el cromosoma 17 se observa con frecuencia en varios cánceres humanos.
- El gen p53, ubicado en el cromosoma 17p13.1, es un gen supresor de tumores crítico.
- Estudios anteriores han indicado la participación del cromosoma 17 en el desarrollo del cáncer de mama.
Objetivo del estudio:
- Identificar y mapear regiones de LOH en el cromosoma 17 en una gran cohorte de pacientes con cáncer de mama.
- Para investigar la relación entre LOH en loci específicos en el cromosoma 17 y el cáncer de mama.
- Explorar el papel potencial de un gen regulador telomérico al p53 en la patogénesis del cáncer de mama.
Principales métodos:
- Análisis de ADN de tumores emparejados y muestras de leucocitos sanguíneos de pacientes con cáncer de mama.
- Análisis de microsatélite para detectar la pérdida de heterocigosidad en el cromosoma 17.
- Análisis cuantitativo de la expresión del ARNm p53.
Principales resultados:
- Alta frecuencia de LOH confirmada en el cromosoma 17p.
- Un tercio de los tumores informativos mostraron pérdida de alelos en el locus THH59 en el brazo largo.
- Dos regiones distintas de LOH identificadas en el brazo corto: 17p13-3 y 17p13-1 (probablemente con p53).
- La región 17p13-3 mostró una frecuencia significativamente mayor de LOH en comparación con 17p13-1.
- No se observó correlación entre las pérdidas de alelos en los dos sitios.
- LOH en 17p13-3 se asoció con la sobreexpresión del ARNm p53.
Conclusiones:
- LOH en 17p13-3 es común en el cáncer de mama y está relacionado con la sobreexpresión del ARNm p53.
- Un gen regulador de aproximadamente 20 megabasas telomérico a p53 en 17p13-3 parece estar involucrado en el cáncer de mama.
- Las lesiones en este gen regulador pueden desempeñar un papel importante en la mayoría de los casos de cáncer de mama.
Más Videos Relacionados
08:15gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
Published on: October 6, 2014
06:29Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland
Published on: June 7, 2019
Videos de Conceptos Relacionados
02:23Abnormal Proliferation
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
01:33Cancer-Critical Genes I: Proto-oncogenes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
01:20The Retinoblastoma Gene
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
01:33Cancer-Critical Genes I: Proto-oncogenes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...