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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Published on: May 3, 2018
El análisis global de los sitios de fosforilación del sustrato Cdk1 proporciona información sobre la evolución de la
Liam J Holt1, Brian B Tuch, Judit Villén
1Departments of Physiology and Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
Science (New York, N.Y.)
|September 26, 2009
Resumen
Se estudiaron los mecanismos de control del ciclo celular mediante el análisis de los sitios de fosforilación de la cinasa dependiente de ciclina Cdk1 en la levadura. La mayoría de los sitios no se conservan, se desplazan en regiones desordenadas, lo que sugiere una rápida evolución de los circuitos de señalización de la kinasa.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
- Biología evolutiva Biología evolutiva.
Sus antecedentes:
- El control del ciclo celular es fundamental para la función celular y el desarrollo del organismo.
- Las cinasas dependientes de ciclina (CDKs) juegan un papel crucial en la regulación del ciclo celular.
- Comprender la evolución de la regulación de la CDK proporciona información sobre la complejidad celular.
Objetivo del estudio:
- Para investigar la conservación evolutiva y la dinámica posicional de los sitios de fosforilación de CDK.
- Explorar los mecanismos por los cuales la fosforilación regula la función e interacciones de las proteínas.
- Para entender la evolución de las redes de señalización de la kinasa.
Principales métodos:
- Se empleó espectrometría de masas cuantitativa para identificar sitios de fosforilación de CDK in vivo.
- La inhibición química específica de Cdk1 se utilizó para mapear sus sustratos.
- Se realizó un análisis genómico comparativo de sustratos CDK a través del linaje de ascomicetos.
Principales resultados:
- Se identificaron 547 sitios de fosforilación en 308 sustratos de Cdk1 en Saccharomyces cerevisiae.
- La posición de la mayoría de los sitios de fosforilación no se conserva evolutivamente.
- Los sitios de fosforilación se agrupan y cambian dentro de regiones de proteínas desordenadas en rápida evolución.
Conclusiones:
- La regulación de la función proteica mediada por la fosforilación a menudo se basa en una simple interrupción o mejora no específica de las interacciones proteína-proteína.
- La ganancia o pérdida de sitios de fosforilación en regiones dinámicas puede impulsar la evolución de las vías de señalización de la kinasa.
- Este estudio proporciona un marco para comprender la evolución de la regulación del ciclo celular a través de modificaciones post-traducionales.
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