Search research articles

ACERCA DE JoVE

Visión General Liderazgo Blog Centro de Ayuda JoVE

AUTORES

Proceso de Publicación Consejo Editorial Alcance y Políticas Revisión por Pares Preguntas Frecuentes Enviar

BIBLIOTECARIOS

Testimonios Suscripciones Acceso Recursos Consejo Asesor de Bibliotecas Preguntas Frecuentes

INVESTIGACIÓN

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archivo

EDUCACIÓN

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Centro de Recursos para Profesores Sitio de Profesores

Términos y Condiciones de Uso

Política de Privacidad

Videos de Conceptos Relacionados

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por

Same author

Acute Lymphoblastic Leukemia in a Child Presenting Primarily with Priapism.

Journal of Indian Association of Pediatric Surgeons·2020

Same author

Lyse-Reseal Erythrocytes for Transfection of Plasmodium falciparum.

Scientific reports·2019

Same author

Management and outcome of a variant of posadas fracture in an adolescent patient: A case report.

Trauma case reports·2019

Same author

Does interaction of monoclonal antibody charge variants with VEGF-A and ELISA reagents affect its quantification?

Analytical biochemistry·2019

Same author

Groundnut <i>AhcAPX</i> conferred abiotic stress tolerance in transgenic banana through modulation of the ascorbate-glutathione pathway.

Physiology and molecular biology of plants : an international journal of functional plant biology·2019

Same author

Understanding the penetrance of intrinsic protein disorder in rotavirus proteome.

International journal of biological macromolecules·2019

Same journal

Decoding Galectin-Glycan Recognition with <sup>19</sup>F-Tagged Lectins: from Simple Glycans to the Cellular Glycocalyx.

Journal of the American Chemical Society·2026

Same journal

Open- and Closed-Shell Roles of Sensitizer and Annihilator in Pseudo-Single Component Mixtures for Upconversion.

Journal of the American Chemical Society·2026

Same journal

Pressure-Induced Superconductivity at 15 K in van-der-Waals Ferroelectric CuInP<sub>2</sub>S<sub>6</sub>.

Journal of the American Chemical Society·2026

Same journal

Carbene Analogues of Group 15: Reduction of s-Hydrindacene-Based Chloropnictogenium Ions To Access an Antimony Hydride Monocation and a Trinuclear Bismuth Dication.

Journal of the American Chemical Society·2026

Same journal

Chiral-Ligand-Modulated Nickel-Catalyzed Stereoselective Radical Migratory C2-Arylation of Carbohydrates.

Journal of the American Chemical Society·2026

Same journal

Coordination-Constraint-Driven Enhanced Chirality Induction in Perovskite Quantum Dot Solids.

Journal of the American Chemical Society·2026

Ver todos los artículos relacionados

Search research articles

Video Experimental Relacionado

Updated: Jun 20, 2026

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

Interferencia condicional del ARN mediada por la ribozima alostérica.

Deepak Kumar¹, Chung-Il An, Yohei Yokobayashi

¹University of California, Davis, Department of Biomedical Engineering 451 Health Sciences Drive, Davis, California 95616, USA.

Journal of the American Chemical Society

|October 1, 2009

Resumen

Este resumen es generado por máquina.

Los investigadores desarrollaron un nuevo sistema de ARN para el control preciso del silenciamiento de genes en las células de mamíferos. Este método de interferencia de ARN inducible químicamente (RNAi) ofrece un enfoque modular y adaptable para regular la expresión génica.

Más Videos Relacionados

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

Videos de Experimentos Relacionados

Last Updated: Jun 20, 2026

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

Área de la Ciencia:

Biología Molecular Biología Molecular
Biotecnología La biotecnología es la biotecnología.
Regulación genética Reglamento genético.

Sus antecedentes:

La interferencia condicional de ARN (RNAi) permite el silenciamiento controlado de genes.
Los métodos existentes a menudo requieren modificaciones genéticas complejas, lo que limita su aplicación.
Existe la necesidad de sistemas más simples y químicamente inducibles de RNAi.

Objetivo del estudio:

Diseñar una nueva arquitectura de ARN para la inducción química de RNAi en las células de mamíferos.
Para crear un sistema compacto y modular para el silenciamiento condicional de genes.
Para habilitar sistemas RNAi que puedan responder a varias señales moleculares.

Principales métodos:

Diseñó una nueva construcción de ARN que combina una ribozima alostérica inducible a fármacos y un análogo de un precursor de microARN.
Utilizó la inducción química para la activación del ARNi en células de mamíferos.
Demostró la modularidad del diseño del ARN para la adaptación potencial.

Principales resultados:

Desarrolló con éxito un sistema de RNAi inducible químicamente en células de mamíferos.
La nueva arquitectura de ARN proporciona un diseño compacto y modular.
El sistema es adaptable para detectar y responder a varias moléculas a través de aptamers de ARN.

Conclusiones:

El nuevo diseño de ARN permite un control químico preciso sobre el silenciamiento de genes.
Este sistema modular facilita la construcción de aplicaciones versátiles de RNAi condicional.
El enfoque ofrece una herramienta prometedora para regular la expresión génica en investigación y potencialmente terapéutica.