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La fosforilación del complejo generador de microARN humano media la señalización MAPK/Erk

Zain Paroo¹, Xuecheng Ye, She Chen

¹Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA.

|October 7, 2009

Resumen

La vía MAPK / Erk regula la producción de microRNA (miRNA) mediante la fosforilación de TRBP, mejorando la biogénesis del miRNA. Esta señalización controla el crecimiento celular y los niveles de miRNA supresor de tumores.

Área de la Ciencia:

Biología Molecular Biología Molecular
La señalización celular de las células.

Sus antecedentes:

Los microARN (miARN) son reguladores cruciales de los procesos biológicos y de las enfermedades.
Comprender la regulación de la vía del miRNA es vital para investigar los mecanismos celulares.

Objetivo del estudio:

Para dilucidar los mecanismos reguladores del complejo generador de miRNA humano.
Para identificar el papel de la proteína quinasa activada por mitógeno (MAPK) Erk en la producción de miRNA.

Principales métodos:

Investigó la composición del complejo generador de miRNA utilizando isoformas Dicer y fosfo-TRBP.
Se examinó el efecto de la fosforilación de TRBP por MAPK/Erk en la producción de miRNA.
Se evaluó el impacto de la señalización MAPK/Erk en los perfiles de miRNA en respuesta al suero y al PMA.

Principales resultados:

El complejo generador de miRNA comprende Dicer y fosfo-TRBP.
La fosforilación de TRBP por MAPK/Erk mejora la producción de miRNA mediante la estabilización del complejo.
La señalización mitogénica y la promoción tumoral implican la fosforilación de TRBP, alterando los niveles de miRNA promotor del crecimiento y supresor del tumor.

Conclusiones:

La vía MAPK/Erk regula directamente la maquinaria del miRNA.
Las vías de señalización se dirigen a la vía del miRNA para mediar respuestas biológicas.
La fosforilación del TRBP es un mecanismo clave que vincula la señalización MAPK/Erk con la biogénesis del miRNA.

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