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Studying the Integration of Adult-born Neurons
Published on: March 25, 2011
El gen de la cadena A de PDGF es expresado por las neuronas de mamíferos durante el desarrollo y en la madurez
1Department of Medicine, Washington University Medical Center, Jewish Hospital, St. Louis, Missouri 63110.
Cell
|January 11, 1991
Resumen
Las neuronas expresan ampliamente el Factor de Crecimiento A Derivado de Plaquetas (PDGF A) en el sistema nervioso de los mamíferos. Esto sugiere que las neuronas juegan un papel clave en el control del desarrollo y la proliferación de las células gliales.
Área de la Ciencia:
- La neurociencia es la neurociencia.
- Biología del desarrollo Biología del desarrollo.
- Biología celular Biología celular.
Sus antecedentes:
- El factor de crecimiento derivado de plaquetas (PDGF) está implicado en el desarrollo del sistema nervioso central de los mamíferos.
- El papel preciso y la localización del PDGF A en el desarrollo neuronal requieren más investigación.
Objetivo del estudio:
- Para investigar la localización y los patrones de expresión del mRNA y la proteína de la cadena A del PDGF en el sistema nervioso de los mamíferos.
- Comprender la regulación del desarrollo y la especificidad tisular de la expresión de PDGF A.
Principales métodos:
- Se empleó la hibridación in situ para detectar el ARNm de cadena A del PDGF.
- La tinción por inmunoperoxidasa se utilizó para identificar la proteína inmunorreactiva PDGF A.
Principales resultados:
- Se encontraron altos niveles de mRNA y proteína de cadena A de PDGF en neuronas de ratón embrionarias y adultas en todo el sistema nervioso central y periférico.
- PDGF Una expresión está regulada en el desarrollo y es específica del tejido.
- Las células gliales mostraron señales variables y más débiles para el PDGF A en comparación con las neuronas.
Conclusiones:
- Las neuronas expresan ampliamente PDGF A, en contraste con la distribución de los factores neurotróficos conocidos.
- La expresión neuronal generalizada de PDGF A sugiere un papel significativo para las neuronas en la regulación de la proliferación y diferenciación de las células gliales in vivo.
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