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Videos de Conceptos Relacionados

Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...

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An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
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La unión combinatoria predice la actividad cis-reguladora espacio-temporal.

Robert P Zinzen1, Charles Girardot, Julien Gagneur

  • 1European Molecular Biology Laboratory, D-69117 Heidelberg, Germany.

Nature
|November 6, 2009
PubMed
Resumen
Este resumen es generado por máquina.

Los científicos desarrollaron un nuevo método para predecir los patrones de expresión génica utilizando datos de unión del factor de transcripción. Este enfoque decodifica con precisión el panorama regulatorio durante el desarrollo sin conocimiento previo de la función de los factores.

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Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

Área de la Ciencia:

  • Biología del desarrollo Biología del desarrollo.
  • La genómica es la genómica.
  • Biología computacional Biología computacional.

Sus antecedentes:

  • Los patrones precisos de expresión génica son cruciales para el desarrollo, controlados por factores de transcripción que se unen a los módulos cis-reguladores.
  • Los datos de ocupación del factor de transcripción en todo el genoma están disponibles, pero la decodificación del panorama regulatorio sigue siendo un desafío.

Objetivo del estudio:

  • Desarrollar un nuevo enfoque basado en datos para predecir la actividad cis-reguladora espacio-temporal.
  • Para crear un atlas de alta resolución de módulos cis-reguladores durante el desarrollo del mesodermo de Drosophila.

Principales métodos:

  • Se utilizan datos de actividad de unión y potenciador del factor de transcripción in vivo.
  • Máquinas vectoriales de soporte entrenadas que utilizan perfiles de unión de módulos cis-reguladores conocidos para predecir patrones de expresión.
  • Se emplean ensayos de reporter transgénico in vivo para validar las predicciones.

Principales resultados:

  • Generó un atlas detallado de los módulos cis-reguladores y su ocupación temporal.
  • Predijo con éxito cinco patrones de expresión espacio-temporal con alta precisión.
  • Se observó una plasticidad inesperada en la unión del factor de transcripción que conduce a resultados de expresión similares.

Conclusiones:

  • El nuevo enfoque predice con precisión la actividad cis-reguladora a partir de datos de unión al factor de transcripción.
  • Este método es ampliamente aplicable ya que no requiere conocimiento previo de la afinidad, función o expresión de la secuencia de factores de transcripción.
  • Los hallazgos revelan plasticidad en la unión del factor de transcripción durante el desarrollo.