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La ocurrencia generalizada de las ribozimas de autoclave
Chiu-Ho T Webb1, Nathan J Riccitelli, Dana J Ruminski
1Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697 USA.
Science (New York, N.Y.)
|December 8, 2009
Resumen
Los ribozimas similares al virus delta de la hepatitis (VHD), un tipo de ARN autoclasificado, están muy extendidos en diversos organismos. Estas abundantes ribozimas sugieren funciones biológicas significativas, pero aún inexploradas.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- ARN Biología Biología ARN
- La genómica es la genómica.
Sus antecedentes:
- El virus delta de la hepatitis (VHD) y las ribozimas de la proteína 3 de unión al elemento de poliadenilación en el citoplasma (CPEB3) son ARN autoclivantes.
- Comparten una estructura de doble pseudo-nudo anidado conservado con una mínima conservación de la secuencia.
Objetivo del estudio:
- Para identificar nuevas secuencias que forman el pliegue de la ribozima parecido al HDV.
- Investigar la distribución y la actividad biológica de estos ribozimas en la naturaleza.
Principales métodos:
- Se emplearon búsquedas computacionales basadas en estructuras secundarias para identificar posibles secuencias de ribozimas.
- Se utilizaron ensayos in vitro para confirmar la actividad de auto-clivado de las secuencias identificadas.
- Se realizaron búsquedas de homología de secuencias para expandir el descubrimiento a través de varios taxones.
Principales resultados:
- Se descubrieron ribozimas activas similares al HDV en organismos marinos, nematodos, artrópodos, bacterias y virus de insectos.
- Estos ribozimas también se encontraron en plantas, hongos y un eucariota unicelular.
- Se observaron diferencias de expresión y actividad de auto-escisión en Anopheles gambiae durante las primeras etapas de desarrollo.
Conclusiones:
- Las ribozimas similares al HDV están ampliamente distribuidas en toda la naturaleza.
- La abundancia y la variada expresión de estos ARN autoclivantes sugieren funciones biológicas diversas e importantes.
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