Search research articles

ACERCA DE JoVE

Visión General Liderazgo Blog Centro de Ayuda JoVE

AUTORES

Proceso de Publicación Consejo Editorial Alcance y Políticas Revisión por Pares Preguntas Frecuentes Enviar

BIBLIOTECARIOS

Testimonios Suscripciones Acceso Recursos Consejo Asesor de Bibliotecas Preguntas Frecuentes

INVESTIGACIÓN

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archivo

EDUCACIÓN

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Centro de Recursos para Profesores Sitio de Profesores

Términos y Condiciones de Uso

Política de Privacidad

Videos de Conceptos Relacionados

Transmission Electron Microscopy

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...

Overview of Electron Microscopy

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

Photoelectric Effect

Photoelectric Effect

When light of a particular wavelength strikes a metal surface, electrons are emitted. This is called the photoelectric effect. The minimum frequency of light that can cause such emission of electrons is called the threshold frequency, which is specific to the metal. Light with a frequency lower than the threshold frequency, even if it is of high intensity, cannot initiate the emission of electrons. However, when the frequency is higher than the threshold value, the number of electrons ejected...

Overview of Microscopy Techniques

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por

Same author

Ultrafast Dynamics of Porphyrins in the Condensed Phase: II. Zinc Tetraphenylporphyrin<sup>†</sup>.

The journal of physical chemistry. A·2025

Same author

Influence of Photoemission Geometry on Timing and Efficiency in 4D Ultrafast Electron Microscopy.

Chemphyschem : a European journal of chemical physics and physical chemistry·2025

Same author

Delineation of the impact on temporal behaviors of off-axis photoemission in an ultrafast electron microscope.

The Review of scientific instruments·2024

Same author

Pulsed-beam transmission electron microscopy and radiation damage.

Micron (Oxford, England : 1993)·2023

Same author

Low repetition-rate, high-resolution femtosecond transmission electron microscopy.

The Journal of chemical physics·2022

Same author

Single-photoelectron collection efficiency in 4D ultrafast electron microscopy.

Physical chemistry chemical physics : PCCP·2022

Search research articles

Video Experimental Relacionado

Updated: Jun 17, 2026

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

Published on: December 3, 2012

Microscopía electrónica de campo cercano inducida por fotones.

Brett Barwick¹, David J Flannigan, Ahmed H Zewail

¹Physical Biology Center for Ultrafast Science and Technology, Arthur Amos Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena, California 91125, USA.

|December 18, 2009

Resumen

La microscopía electrónica de campo cercano inducida por fotones (PINEM) permite obtener imágenes de campos electromagnéticos evanescentes con pulsos de electrones. Esta técnica logra una resolución a escala atómica, visualizando las interacciones luz-materia a nanoescala.

Más Videos Relacionados

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging

Published on: November 25, 2009

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

Published on: October 23, 2018

Videos de Experimentos Relacionados

Last Updated: Jun 17, 2026

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

Published on: December 3, 2012

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging

Published on: November 25, 2009

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

Published on: October 23, 2018

Área de la Ciencia:

Ciencia de los materiales Ciencia de los materiales.
La óptica cuántica es una óptica cuántica.
Microscopía de electrones por microscopía electrónica.

Sus antecedentes:

La microscopia óptica de campo cercano ofrece una resolución de subdifracción, pero carece de capacidades a escala atómica.
La microscopía electrónica proporciona una resolución atómica, pero no puede obtener imágenes de campos ópticos evanescentes.

Objetivo del estudio:

Desarrollar una técnica que combine las interacciones de electrones y fotones para la obtención de imágenes a nanoescala.
Para lograr imágenes a escala atómica de campos electromagnéticos evanescentes utilizando pulsos de electrones.

Principales métodos:

Desarrolló la microscopía electrónica de campo cercano inducida por fotones (PINEM).
Utilizó la superposición espacio-temporal de paquetes de electrones femtosegundos e impulsos ópticos en nanoestructuras.
Filtración de energía empleada de electrones relativistas (200 keV) después de la absorción de fotones.

Principales resultados:

Se ha demostrado la absorción directa de los cuantos fotónicos (nhω) por los electrones relativistas.
Logró imágenes espaciales directas de las distribuciones del campo eléctrico de campo cercano.
Se obtuvo una resolución temporal de femtosegundos de campos ópticos y se mapeó la dependencia de polarización.

Conclusiones:

PINEM permite imágenes espaciotemporales directas de campos localizados a nanoescala.
La técnica visualiza fenómenos en fotónica, plasmónica y nanoestructuras con un detalle sin precedentes.
Este avance cierra la brecha entre la microscopía óptica y la electrónica para estudios avanzados de materiales y biológicos.