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Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
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Un papel para la Enzima Viejo Amarillo en la biosíntesis de alcaloides de ergot
Johnathan Z Cheng1, Christine M Coyle, Daniel G Panaccione
1Massachusetts Institute of Technology, Department of Chemistry, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
Journal of the American Chemical Society
|January 28, 2010
Resumen
Los hongos filamentosos producen alcaloides de ergot con diversos efectos. Los investigadores identificaron una enzima clave en el Aspergillus fumigatus que reduce un intermediario, lo que permite la formación del anillo D. del alcaloide del ergot.
Área de la Ciencia:
- La bioquímica es la bioquímica.
- La micología de la micología.
- Síntesis de Productos Naturales.
Sus antecedentes:
- Los alcaloides de ergot son metabolitos secundarios farmacológicamente significativos de los hongos filamentosos.
- Las vías biosintéticas completas para los alcaloides de ergot permanecen incompletamente entendidas.
- La comprensión de la biosíntesis es crucial para las posibles aplicaciones farmacéuticas.
Objetivo del estudio:
- Para dilucidar un paso clave en la biosíntesis de los alcaloides de ergot.
- Para identificar la enzima responsable de una reacción de reducción específica en la vía.
- Para entender la formación del anillo D en la estructura de los alcaloides de ergot.
Principales métodos:
- Análisis del grupo de genes del ergot Aspergillus fumigatus.
- Caracterización bioquímica de una Enzima Amarilla Vieja homóloga.
- Pruebas enzimáticas para determinar la especificidad del sustrato y la formación del producto.
Principales resultados:
- Se identificó un homólogo de la enzima amarilla vieja de Aspergillus fumigatus.
- Esta enzima cataliza la reducción del alqueno alfa, beta insaturado del aldehído de canoclavina-I.
- El producto de reducción, dihidrocanoclavina aldehído, facilita la formación del anillo D a través de la ciclización intramolecular.
Conclusiones:
- La enzima identificada juega un papel crítico en la biosíntesis de los alcaloides de ergot.
- Este hallazgo avanza en la comprensión de la formación del marco estructural del alcaloide ergot.
- La elucidación de este paso proporciona información sobre la síntesis de productos naturales por hongos.
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