Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Forces Acting on Chromosomes02:11

Forces Acting on Chromosomes

During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis. 
Microtubules and motor proteins exert two types of forces on...
Forces Acting on Chromosomes02:11

Forces Acting on Chromosomes

During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis. 
Microtubules and motor proteins exert two types of forces on...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Anaphase A and B01:39

Anaphase A and B

Microtubules form through the end-to-end polymerization of tubulin heterodimers. Kinetochore microtubules originate from the spindle poles, and their plus-ends connect with the kinetochores on sister-chromatids. Ndc80 protein complexes, present on the kinetochore, form low-affinity links with the plus end of these kinetochore microtubules.
Plus-end depolymerization releases tubulin heterodimers from the terminal region of the microtubule. As tubulin subunits are lost, the Ndc80 complexes detach...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Coordinated chromosome motion emerges from mechanical coupling mediated by the physical spindle environment.

Molecular biology of the cell·2026
Same author

The Role of Transient Crosslinks in the Chromatin Search Response to DNA Damage.

International journal of molecular sciences·2025
Same author

Different relative scalings between transient forces and thermal fluctuations tune regimes of dynamic clustering.

Physical review. E·2025
Same author

The centromere bottlebrush requires a multi-microtubule attachment.

Molecular biology of the cell·2025
Same author

Centromeres are stress-induced fragile sites.

Current biology : CB·2025
Same author

Closing the loops: chromatin loop dynamics after DNA damage.

Nucleus (Austin, Tex.)·2024

Video Experimental Relacionado

Updated: Jun 16, 2026

Live Cell Imaging of Chromosome Segregation During Mitosis
06:39

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Hacia la construcción de una máquina de segregación cromosómica.

Kerry Bloom1, Ajit Joglekar

  • 1Department of Biology, 622 Fordham Hall, CB3280, University of North Carolina at Chapel Hill, North Carolina 27599, USA. kerry_bloom@unc.edu

Nature
|January 30, 2010
PubMed
Resumen
Este resumen es generado por máquina.

Todos los organismos deben empaquetar y segregar su ADN durante la división celular. Comprender estos complejos mecanismos de envasado y segregación del genoma es crucial, ya que varían significativamente entre las especies.

Más Videos Relacionados

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
07:48

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Published on: October 11, 2022

Videos de Experimentos Relacionados

Last Updated: Jun 16, 2026

Live Cell Imaging of Chromosome Segregation During Mitosis
06:39

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
07:48

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Published on: October 11, 2022

Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • Genética La genética.
  • Biología celular Biología celular.

Sus antecedentes:

  • La división celular requiere la replicación precisa, el empaquetado y la segregación de grandes cantidades de ADN.
  • Si bien los mecanismos de replicación del ADN son bien conocidos, los procesos de empaquetado y segregación del genoma siguen siendo menos claros.
  • Estos procesos son fundamentales para toda la vida, desde las bacterias hasta los seres humanos.

Objetivo del estudio:

  • Explorar los complejos mecanismos de envasado y segregación del ADN durante la división celular.
  • Para resaltar la variabilidad de estos mecanismos en diferentes organismos.
  • Para subrayar la investigación en curso para comprender la organización del genoma.

Principales métodos:

  • Análisis comparativo de genómica para identificar mecanismos conservados y variables.
  • Técnicas de biología molecular para estudiar las interacciones ADN-proteína.
  • Imágenes celulares para visualizar la organización del genoma durante la división.

Principales resultados:

  • Identificó diversas estrategias empleadas por los organismos para el empaquetado y la segregación del ADN.
  • Se destacan las principales familias de proteínas involucradas en la organización del genoma.
  • Se demostraron diferencias significativas en los mecanismos incluso entre especies estrechamente relacionadas.

Conclusiones:

  • El empaquetado y la segregación del genoma son procesos complejos y altamente variables esenciales para la división celular.
  • Se necesita más investigación para dilucidar completamente los diversos mecanismos moleculares involucrados.
  • Comprender estos mecanismos es fundamental para campos que van desde la biología del desarrollo hasta la investigación de enfermedades.