Video Experimental Relacionado
Updated: Apr 16, 2026
14:32
Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates
Published on: February 27, 2016
8.8K
La histona desacetilasa Sirt6 regula la homeostasis de la glucosa a través de Hif1alpha, que es el receptor de la
Lei Zhong1, Agustina D'Urso, Debra Toiber
1The Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston, MA 02114, USA.
Cell
|February 10, 2010
Resumen
El estudio revela que la proteína SIRT6 controla los niveles de glucosa mediante la regulación de los genes glicolíticos. La deficiencia de SIRT6 conduce a la hipoglucemia, afectando la homeostasis de la glucosa y el tratamiento de la enfermedad metabólica.
Área de la Ciencia:
- La bioquímica es la bioquímica.
- Biología Molecular Biología Molecular
- Genética La genética.
Sus antecedentes:
- SIRT6 es una desacetilasa dependiente de NAD (((+) involucrada en el metabolismo y la resistencia al estrés.
- Los ratones con deficiencia de SIRT6 presentan hipoglucemia letal, pero el mecanismo subyacente es desconocido.
Objetivo del estudio:
- Para aclarar el papel de SIRT6 en la homeostasis de la glucosa.
- Investigar el mecanismo por el cual la deficiencia de SIRT6 causa hipoglucemia.
Principales métodos:
- Análisis de la histona desacetilasa. ensayos de la histona desacetilasa.
- Análisis de la expresión génica.
- Análisis de la absorción y el metabolismo de la glucosa celular en células y ratones con deficiencia de SIRT6.
Principales resultados:
- SIRT6 actúa como una histona H3K9 desacetilasa, regulando la expresión génica glicolítica.
- SIRT6 funciona como un corepresor del factor 1-alfa inducible por hipoxia (Hif1alpha).
- La deficiencia de SIRT6 conduce a un aumento de la actividad Hif1alpha, una mayor glucólisis, una mayor absorción de glucosa y una reducción de la respiración mitocondrial.
Conclusiones:
- SIRT6 es un regulador clave de la homeostasis de la glucosa a través de su control de la expresión génica glucolítica.
- El papel de SIRT6 en la regulación de Hif1alfa y la glucólisis ofrece objetivos terapéuticos potenciales para enfermedades metabólicas como la diabetes y la obesidad.
Más Videos Relacionados
Videos de Conceptos Relacionados
01:58Cell Specific Gene Expression
17.4K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
17.4K
01:19Regulation of Metabolism
12.5K
Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
12.5K
01:16Hormones Regulating Blood Glucose
9.0K
Insulin is released by beta cells of the pancreas when blood glucose levels are high. It facilitates glucose absorption and utilization in insulin-dependent cells with insulin receptors on their plasma membranes. Insulin promotes glucose uptake by increasing the number of glucose transport proteins in the cell membrane, allowing glucose to enter the cell. As a result, glucose utilization and ATP production are enhanced.
In addition to accelerating glucose uptake and utilization, insulin has...
In addition to accelerating glucose uptake and utilization, insulin has...
9.0K
01:02Glucose Homeostasis: Regulation of Blood Glucose
5.5K
Carbohydrates consumed through foods are converted into glucose, a crucial energy source for the body. In the prandial state, high blood glucose levels stimulate the secretion of insulin from the pancreas. Insulin inhibits hepatic glucose production and stimulates glucose uptake and metabolism by muscle and adipose tissue. The excess glucose is converted into glycogen and stored in the liver and muscles.
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
5.5K
01:27Glucose Homeostasis: Pancreatic Islets and Insulin Secretion
3.2K
The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are...
Insulin and C-peptide are...
3.2K
01:28Insulin: The Receptor and Signaling Pathways
6.4K
Insulin action is mediated through a receptor tyrosine kinase, akin to the IGF-1 receptor. The number of receptors per cell varies significantly, from 40 on erythrocytes to 300,000 on adipocytes and hepatocytes. The insulin receptor consists of linked α/β subunit dimers, forming a heterotetramer glycoprotein with two extracellular α subunits and two β subunits spanning the membrane. The α subunits inhibit the inherent tyrosine kinase activity of the β subunits, but...
6.4K