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El complejo PAF1 humano actúa en el alargamiento de la transcripción de la cromatina de forma independiente y
Jaehoon Kim1, Mohamed Guermah, Robert G Roeder
1The Rockefeller University, New York, NY 10065, USA.
Cell
|February 25, 2010
Resumen
El complejo PAF1 (PAF1C) facilita directamente el alargamiento de la transcripción de la cromatina. Funciona sinérgicamente con el factor de alargamiento SII / TFIIS, revelando un nuevo papel para PAF1C en la regulación génica.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Reglamento genético Reglamento genético.
- Dinámica de la cromatina Dinámica de la cromatina Dinámica de la cromatina Dinámica de la cromatina Dinámica de la cromatina Dinámica de la cromatina Dinámica de la cromatina Dinámica de la cromatina Dinámica de la cromatina Dinámica de la cromatina Dinámica de la cromatina
Sus antecedentes:
- El complejo PAF1 (PAF1C) está vinculado a la transcripción, pero su papel directo en la transcripción de la cromatina no está claro.
- Estudios anteriores se centraron en la participación de PAF1C en la modificación de histonas, no en la facilitación directa de la transcripción.
Objetivo del estudio:
- Investigar el papel directo del PAF1C humano (hPAF1C) en el alargamiento de la transcripción a partir de una plantilla de cromatina.
- Para aclarar el mecanismo de la función de hPAF1C y su interacción con otros factores de transcripción.
Principales métodos:
- Sistema de transcripción de ARN polimerasa II definido bioquímicamente.
- Pruebas de muestras de cromatina recombinante.
- Análisis de las interacciones de hPAF1C y el factor de alargamiento SII/TFIIS.
Principales resultados:
- hPAF1C facilita directamente el alargamiento de la transcripción dependiente del activador (p53) y la histona acetiltransferasa (p300) de la cromatina.
- Se identificó una nueva función de hPAF1C distinta de la ubicuidad y la metilación de histonas.
- Se observó una fuerte sinergia entre hPAF1C y el factor de alargamiento SII/TFIIS, mediada por interacciones directas y unión cooperativa a la ARN polimerasa II.
Conclusiones:
- hPAF1C posee una función distinta en promover directamente el alargamiento de la transcripción de la cromatina.
- Se reveló un mecanismo molecular para la acción cooperativa de hPAF1C y SII/TFIIS en la transcripción de la cromatina.
- Estos hallazgos avanzan en la comprensión de la cooperación de los factores de transcripción en la expresión génica.
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