Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Membrane Fluidity01:23

Membrane Fluidity

Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.
Membrane Fluidity01:26

Membrane Fluidity

Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is a relatively...
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
Role of ER in the Secretory Pathway01:17

Role of ER in the Secretory Pathway

Eukaryotic cells have a special pathway that enables communication between various intracellular membrane-bound compartments and also with the extracellular environment. This pathway is termed as the secretory pathway.
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
Lipid-derived Compounds in the Human Body01:31

Lipid-derived Compounds in the Human Body

Fats and lipids are crucial components in the human body. Some lipid-derived compounds, such as fat-soluble vitamins, eicosanoids, lipoproteins, and glycolipids, also play unique roles to support various  biological processes .
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin, delayed...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

A tumor profiling resource for ovarian cancer: insights into chemotherapy-driven heterogeneity and personalized treatment strategy.

Nature communications·2026
Same author

Imaging mass cytometry reveals functional and immunological changes during type 1 diabetes progression in human pancreata.

Nature metabolism·2026
Same author

Primary cilium disassembly - from mechanisms to roles in physiology and disease.

Journal of cell science·2026
Same author

A lipidomics roadmap: from basic research to societal challenges.

Nature communications·2026
Same author

Spatially-resolved single-cell imaging of melanoma brain metastases identifies localized immune patterns predictive of immune checkpoint blockade response.

Neuro-oncology·2026
Same author

Absolute quantification of fluorescent protein fusions by mass spectrometry.

Protein science : a publication of the Protein Society·2026

Video Experimental Relacionado

Updated: May 9, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
08:59

Defining Substrate Specificities for Lipase and Phospholipase Candidates

Published on: November 23, 2016

Las proteínas de la familia Orm median en la homeostasis de los esfingolipidos.

David K Breslow1, Sean R Collins, Bernd Bodenmiller

  • 1Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 1700 4th Street, San Francisco, California 94158, USA.

Nature
|February 26, 2010
PubMed
Resumen

Los investigadores descubrieron que las proteínas Orm regulan la síntesis de esfingolipidos. La fosforilación controla esta regulación, vinculando la homeostasis de los esfingolipidos con el riesgo de asma infantil.

Más Videos Relacionados

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
08:49

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Published on: March 14, 2021

Fiber Type and Subcellular-Specific Analysis of Lipid Droplet Content in Skeletal Muscle
11:50

Fiber Type and Subcellular-Specific Analysis of Lipid Droplet Content in Skeletal Muscle

Published on: June 8, 2022

Videos de Experimentos Relacionados

Last Updated: May 9, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
08:59

Defining Substrate Specificities for Lipase and Phospholipase Candidates

Published on: November 23, 2016

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
08:49

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Published on: March 14, 2021

Fiber Type and Subcellular-Specific Analysis of Lipid Droplet Content in Skeletal Muscle
11:50

Fiber Type and Subcellular-Specific Analysis of Lipid Droplet Content in Skeletal Muscle

Published on: June 8, 2022

Área de la Ciencia:

  • Biología celular Biología celular.
  • La bioquímica es la bioquímica.
  • Genética La genética.

Sus antecedentes:

  • Los esfingolipidos son componentes vitales de la membrana y moléculas de señalización.
  • La regulación celular de los niveles de esfingolipidos sigue siendo poco comprendida.
  • Los genes ORM, incluido el humano ORMDL3, están implicados en el asma infantil.

Objetivo del estudio:

  • Para dilucidar la función de los genes ORM en el metabolismo de los esfingolípidos.
  • Para identificar los mecanismos reguladores que controlan la síntesis de esfingolipidos.
  • Para explorar el vínculo entre la desregulación de los esfingolipidos y el asma.

Principales métodos:

  • Cribado genómico funcional en Saccharomyces cerevisiae.
  • Ensayos bioquímicos para caracterizar las interacciones de las proteínas.
  • Análisis de la expresión génica y la fosforilación de proteínas.

Principales resultados:

  • Las proteínas Orm actúan como reguladores negativos de la síntesis de esfingolipidos.
  • Las proteínas Orm forman un complejo con la serina palmitoiltransferasa.
  • La fosforilación de las proteínas Orm modula su actividad inhibidora.
  • Los cambios en la expresión génica ORM o las mutaciones en el sitio de fosforilación interrumpen la homeostasis de los esfingolipidos.

Conclusiones:

  • Las proteínas Orm son mediadores críticos de la homeostasis de los esfingolípidos.
  • La desregulación del metabolismo de los esfingolipidos puede contribuir al asma infantil.
  • Este estudio revela una nueva vía reguladora para la síntesis de esfingolipidos.