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Control de la polaridad del embrión apical-basal de Arabidopsis por factores de transcripción antagónicos

Zachery R Smith¹, Jeff A Long

¹Plant Biology Laboratory, The Salk Institute for Biological Studies, University of California San Diego, La Jolla, California 92037, USA.

|March 2, 2010

Resumen

Los investigadores identificaron genes clave que controlan el desarrollo de los embriones de las plantas. Los genes CLASS III HOMEODOMAIN-LEUCINE ZIPPER (HD-ZIP III) regulan la formación de brotes, mientras que los genes PLETHORA (PLT) controlan el desarrollo de las raíces, mostrando una relación antagónica.

Área de la Ciencia:

Biología del desarrollo vegetal Biología del desarrollo vegetal
La embriogénesis de la arabidopsis
Regulación genética en las plantas.

Sus antecedentes:

Las plantas establecen un eje apical-basal durante la embriogénesis para el patrón de órganos.
Los genes PLETHORA (PLT) son reguladores conocidos del destino de la raíz, pero los reguladores del destino apical / shoot eran desconocidos.
TOPLESS (TPL) es un co-represor de la transcripción involucrado en el desarrollo de las plantas.

Objetivo del estudio:

Para identificar los reguladores maestros del destino apical / tiro en la embriogénesis de Arabidopsis.
Para aclarar las interacciones genéticas entre las vías reguladoras de brotes y raíces.
Para entender el papel de TOPLESS (TPL) en el establecimiento del eje.

Principales métodos:

Análisis genético utilizando mutantes tpl-1 en la especie Arabidopsis.
Análisis de la expresión génica de los genes PLT y HD-ZIP III.
Estudios de expresión errónea para evaluar la función génica en la determinación del destino.

Principales resultados:

Los genes PLT1 y PLT2 son objetivos directos de TOPLESS (TPL).
Los factores de transcripción HOMEODOMAIN-LEUCINE ZIPPER (HD-ZIP III) de clase III se identifican como los reguladores maestros del destino apical. los factores de transcripción HOMEODOMAIN-LEUCINE ZIPPER (HD-ZIP III) también se identifican como los reguladores maestros del destino apical. los factores de transcripción HOMEODOMAIN-LEUCINE ZIPPER (HD-ZIP III) también se identifican como los reguladores maestros del destino apical.
Los genes HD-ZIP III pueden convertir el poste de raíz embrionario en un poste de brote.
Existe una relación antagónica entre los genes PLT y HD-ZIP III en la especificación de polos.

Conclusiones:

Los genes HD-ZIP III son suficientes para conferir el destino del tiro, mientras que los genes PLT confieren el destino de la raíz.
La interacción entre los genes PLT y HD-ZIP III es crucial para establecer los polos de brote y raíz.
TOPLESS (TPL) actúa aguas arriba de los genes PLT en la regulación del destino basal / raíz.

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