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Videos de Conceptos Relacionados

General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

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Video Experimental Relacionado

Updated: Jun 14, 2026

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

Variación en la unión del factor de transcripción entre los seres humanos.

Maya Kasowski1, Fabian Grubert, Christopher Heffelfinger

  • 1Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.

Science (New York, N.Y.)
|March 20, 2010
PubMed
Resumen

Las diferencias en la unión del factor de transcripción (TF) impactan significativamente la expresión génica, contribuyendo a la individualidad humana y la divergencia de especies. Estas variaciones, a menudo vinculadas a cambios genéticos, ofrecen información sobre los procesos evolutivos.

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Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

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Videos de Experimentos Relacionados

Last Updated: Jun 14, 2026

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
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High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
11:25

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Published on: February 11, 2019

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

Área de la Ciencia:

  • La genómica es la genómica.
  • Biología evolutiva Biología evolutiva.
  • Biología Molecular Biología Molecular

Sus antecedentes:

  • La variación de la expresión génica es crucial para la especiación y la diversidad fenotípica.
  • La unión del factor de transcripción (TF) es un mecanismo regulador clave que influye en la expresión génica.

Objetivo del estudio:

  • Investigar las diferencias en todo el genoma en la unión del factor de transcripción entre individuos y especies.
  • Para correlacionar las variaciones de unión a TF con cambios genéticos y diferencias en la expresión génica.

Principales métodos:

  • La inmunoprecipitación de cromatina seguida de secuenciación (ChIP-seq) se utilizó para mapear los sitios de unión.
  • Se analizaron los sitios de unión de la ARN polimerasa II (PolII) y el factor nuclear kappaB (p65) en líneas celulares humanas.
  • Se compararon los patrones de unión PolII de humanos y chimpancés.

Principales resultados:

  • Se observaron diferencias significativas en los sitios de unión de PolII y p65 entre individuos humanos (25% y 7.5%, respectivamente).
  • Las variaciones de unión a TF se asociaron con frecuencia con polimorfismos de un solo nucleótido y variantes estructurales.
  • Las diferencias en la unión de TF a menudo se correlacionan con la expresión génica alterada, lo que indica relevancia funcional.
  • Se encontró una amplia divergencia en la unión de PolII entre humanos y chimpancés.

Conclusiones:

  • La variación de la unión al factor de transcripción es una fuente importante de diferencias entre individuos y especies.
  • Estos hallazgos proporcionan información sobre los fundamentos genéticos de la diversidad fenotípica y la especiación.