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Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
Published on: September 10, 2013
Partición de los tetrámeros de histona H3-H4 durante el ensamblaje de la cromatina dependiente de la replicación del
Mo Xu1, Chengzu Long, Xiuzhen Chen
1Graduate Program, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, People's Republic of China.
Science (New York, N.Y.)
|April 3, 2010
Resumen
Las modificaciones de las histonas, cruciales para la memoria epigenética, se propagan durante la división celular. Este estudio revela que los tetrámeros H3.3-H4 se dividen durante la replicación del ADN, a diferencia de los tetrámeros H3.1-H4, lo que aclara los mecanismos de herencia epigenética.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- La epigenética es la epigenética.
- Biología celular Biología celular.
Sus antecedentes:
- La replicación semiconservadora del ADN asegura la fidelidad genética, pero la propagación de la información epigenética a través de modificaciones histónicas a través de divisiones mitóticas no se entiende completamente.
- Aclarar el patrón de partición del nucleosoma durante la replicación del ADN es esencial para comprender la herencia epigenética.
Objetivo del estudio:
- Para investigar el patrón de partición de los tetrámeros de histona H3.3-H4 y H3.1-H4 durante la replicación del ADN.
- Determinar el papel de la deposición dependiente de la replicación del ADN en la división del tetramero de histona y el mantenimiento de la memoria epigenética.
Principales métodos:
- Análisis in vivo de la división del tetramero de histona (H3.3-H4 y H3.1-H4) durante la replicación del ADN.
- Inhibición de la deposición de histonas dependientes de la replicación del ADN para evaluar su impacto en los eventos de división.
Principales resultados:
- Se observó una división significativa in vivo de los tetrámeros H3.3-H4, mientras que los tetrámeros H3.1-H4 permanecieron en gran medida intactos.
- La inhibición de la deposición dependiente de la replicación redujo sustancialmente la división de las histonas tetrámeras.
- La deposición de H3.3 independiente de la replicación implica la incorporación cooperativa de dos nuevos dímeros H3.3-H4.
Conclusiones:
- La mayoría de los eventos de división de tetrámeros H3-H4 ocurren durante la deposición dependiente de la replicación.
- Las modificaciones epigenéticas en la heterocromatina se mantienen a través de la copia de las histonas vecinas, sin requerir la división H3-H4.
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