Video Experimental Relacionado
Updated: Jun 13, 2026
09:32
Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
Published on: June 15, 2017
Diversificación rápida de los fenotipos de señalización celular por la recombinación de dominio modular
Sergio G Peisajovich1, Joan E Garbarino, Ping Wei
1Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
Science (New York, N.Y.)
|April 17, 2010
Resumen
La recombinación del dominio en las proteínas de señalización celular impulsa la innovación evolutiva. Este estudio muestra que la combinación de dominios de proteínas crea una mayor diversidad en las vías de apareamiento de la levadura que la duplicación génica, lo que conduce a una mayor eficiencia de apareamiento.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología evolutiva Biología evolutiva.
- Biología de Sistemas Biología de Sistemas.
Sus antecedentes:
- Las proteínas de señalización celular poseen estructuras modulares con distintos dominios catalíticos y reguladores.
- Se supone que la recombinación de estos módulos biológicos es un motor significativo de la novedad evolutiva.
Objetivo del estudio:
- Analizar sistemáticamente la diversidad fenotípica que surge de la recombinación de dominios en las vías de señalización celular.
- Para comparar el impacto de la recombinación de dominio en la dinámica de la respuesta de señalización frente a otros mecanismos de variación genética.
Principales métodos:
- Construcción de una biblioteca de 66 recombinantes de dominio quimérico utilizando 11 proteínas de la vía de apareamiento de la levadura.
- Análisis sistemático de la diversidad fenotípica y la dinámica de respuesta de la vía en los recombinantes construidos.
Principales resultados:
- La recombinación de dominio generó una mayor diversidad en la dinámica de respuesta de la vía en comparación con la duplicación de genes, la duplicación de un solo dominio o la duplicación de dos dominios no vinculados.
- Las recombinantes exhibieron fenotipos de apareamiento alterados, y algunas mostraron una mayor eficiencia de apareamiento en comparación con el tipo salvaje.
Conclusiones:
- Los nuevos vínculos entre los dominios de proteínas existentes juegan un papel crucial en la evolución de las redes de proteínas.
- La recombinación de dominio es un mecanismo significativo para generar nuevos comportamientos fenotípicos e impulsar la innovación evolutiva.
Videos de Conceptos Relacionados
01:22Diversity in Cell Signaling Responses
The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity.
Graded and Abrupt Responses
Some signaling systems generate...
Graded and Abrupt Responses
Some signaling systems generate...
01:30Assembly of Signaling Complexes
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
01:28Diversity of Antigen Receptors
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
01:23Overview of Cell Signaling
Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate with the environment.
Cells respond to many types of information, often through receptor proteins positioned on the membrane. For example, skin cells respond to and transmit touch...
Cells respond to many types of information, often through receptor proteins positioned on the membrane. For example, skin cells respond to and transmit touch...
01:23Overview of Cell Signaling
Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate with the environment.
Cells respond to many types of information, often through receptor proteins positioned on the membrane. For example, skin cells respond to and transmit touch...
Cells respond to many types of information, often through receptor proteins positioned on the membrane. For example, skin cells respond to and transmit touch...
02:53Conservative Site-specific Recombination and Phase Variation
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...