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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
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La proteína del andamio Ste5 controla directamente una decisión de apareamiento similar a un interruptor en las
Mohan K Malleshaiah1, Vahid Shahrezaei, Peter S Swain
1Département de Biochimie, Bio-Informatique et Génomique Université de Montréal, C.P. 6128, Succursale centre-ville Montréal, Québec H3C 3J7, Canada.
Nature
|April 20, 2010
Resumen
Las decisiones de apareamiento de las levaduras implican una respuesta similar a un interruptor a las feromonas, controlada por la competencia entre el MAPK Fus3 y la fosfatasa Ptc1.1. Este mecanismo asegura una determinación robusta del cell-fate, incluso con diferentes niveles de proteínas.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- Biología evolutiva Biología evolutiva.
Sus antecedentes:
- Los organismos desarrollan estrategias de apareamiento, incluida la señalización química como las feromonas en la levadura (Saccharomyces cerevisiae).
- Las levaduras utilizan una vía de proteína quinasa activada por mitógeno (MAPK) para interpretar los gradientes de feromonas para el apareamiento.
- La respuesta de apareamiento similar a un interruptor en las levaduras filtra las señales débiles, asegurando el compromiso solo en concentraciones críticas de feromonas.
Objetivo del estudio:
- Para dilucidar los mecanismos moleculares subyacentes a la decisión de apareamiento en forma de interruptor en la levadura.
- Para identificar las proteínas clave y las interacciones que rigen esta rápida decisión del destino celular.
Principales métodos:
- Investigó la competencia entre el MAPK Fus3 y la fosfatasa Ptc1.1.
- Se analizó el estado de fosforilación de la proteína del andamio Ste5.5.
- Examinó la disociación de Fus3 de Ste5 en respuesta a la señalización de feromonas.
Principales resultados:
- La respuesta de apareamiento similar a un interruptor es impulsada por la competencia entre Fus3 y Ptc1 para la fosforilación de Ste5.
- Esta competencia conduce a una rápida disociación de Fus3 de Ste5.5, parecida a una conmutación.
- El circuito Fus3-Ste5-Ptc1 exhibe una ultrasensibilidad, robusta a las variaciones de la concentración de proteínas.
Conclusiones:
- La decisión del destino celular de aparearse se toma al principio de la vía feromónica a través de la competencia Fus3-Ste5-Ptc1.
- Este mecanismo ultrasensible asegura un apareamiento confiable a pesar de la variabilidad biológica.
- Las proteínas del andamio como Ste5 juegan un papel directo en las decisiones del destino celular, con implicaciones para los organismos superiores y enfermedades como el cáncer.
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