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Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
Published on: February 3, 2020
Los pequeños ARN silenciadores en las plantas son modificaciones epigenéticas móviles y directas en las células
Attila Molnar1, Charles W Melnyk, Andrew Bassett
1Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, UK.
Science (New York, N.Y.)
|April 24, 2010
Resumen
Los pequeños ARN (ARNm) se mueven entre los tejidos de las plantas, llevando instrucciones epigenéticas. Este estudio identifica sRNAs móviles que dirigen las modificaciones epigenéticas de todo el genoma en las células receptoras, impactando la defensa de las plantas.
Área de la Ciencia:
- Biología molecular vegetal Biología molecular vegetal
- La epigenética es la epigenética.
- Biología del ARN Biología del ARN
Sus antecedentes:
- El silenciamiento del ARN es un proceso regulador crucial en las plantas.
- Se sabe que las señales móviles transmiten información entre los tejidos vegetales.
- La naturaleza y la función de los ARN móviles aún no se entienden completamente.
Objetivo del estudio:
- Para identificar los ARN pequeños específicos (ARNm) que funcionan como señales silenciadoras móviles en las plantas.
- Para investigar el movimiento de estos ARNs s a través de uniones de injerto.
- Determinar el papel de los ARNs móviles en la dirección de las modificaciones epigenéticas en las células vegetales receptoras.
Principales métodos:
- Experimentos de injerto utilizando Arabidopsis thaliana para crear sistemas de tejidos fuente-receptor.
- Utilizando mutantes deficientes en la biogénesis de ARN pequeño en tejidos de origen o receptores.
- Analizando el movimiento de sRNAs derivados de transgenes y endógenos a través de la unión del injerto.
- Proporcionar evidencia para el papel de los ARNs móviles en la dirección de las modificaciones epigenéticas.
Principales resultados:
- Se descubrió que los ARN pequeños endógenos y derivados de transgenes (ARNs) se mueven de la fuente a los tejidos receptores a través de la unión del injerto.
- La evidencia sugiere que los sRNA móviles de 24 nucleótidos son responsables de dirigir las modificaciones epigenéticas en las células receptoras.
- Los ARNs móviles facilitan la transmisión de las especificaciones epigenéticas dentro de la planta.
Conclusiones:
- Los pequeños ARN móviles (ARNm) actúan como señales cruciales para la transmisión de información epigenética en las plantas.
- Este mecanismo de movimiento de ARNm móvil juega un papel en la defensa del genoma de la planta y las respuestas a los estímulos ambientales.
- La comprensión de la función del ARNm móvil ofrece información sobre la herencia epigenética persistente y la adaptación de las plantas.
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