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A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis
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Evolución adaptativa de un sRNA que controla el desarrollo de Myxococcus.

Yuen-Tsu N Yu1, Xi Yuan, Gregory J Velicer

  • 1Department of Biology, Indiana University, Bloomington, IN 47405, USA.

Science (New York, N.Y.)
|May 22, 2010
PubMed
Resumen

Los ARN pequeños (ARNs) regulan la biología. Un sRNA específico, Pxr, controla el desarrollo en Myxococcus xanthus, y su mutación restauró adaptivamente el desarrollo en cepas tramposas.

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Área de la Ciencia:

  • Microbiología Microbiología.
  • Biología Molecular Biología Molecular
  • Biología evolutiva Biología evolutiva.

Sus antecedentes:

  • Las moléculas de ARN pequeño (ARNm) son reguladores cruciales en los procesos biológicos.
  • La evidencia de la evolución adaptativa en las secuencias de sRNA ha sido limitada.
  • El desarrollo multicelular en los procariotas implica complejas redes reguladoras.

Objetivo del estudio:

  • Identificar y caracterizar un sRNA involucrado en la regulación del desarrollo multicelular en Myxococcus xanthus.
  • Investigar el papel de la evolución del ARNm en la adaptación fenotípica.
  • Para demostrar el impacto directo de los reguladores de ARNm en el cambio evolutivo.

Principales métodos:

  • Identificación de un nuevo sRNA, Pxr, en el Myxococcus xanthus.
  • Análisis funcional del papel de Pxr en la regulación del desarrollo del cuerpo fructífero.
  • Manipulación genética para estudiar el efecto de las mutaciones Pxr en cepas tramposas de M. xanthus.

Principales resultados:

  • Pxr fue identificado como un regulador negativo del desarrollo del cuerpo frutal en M. xanthus.
  • Una mutación espontánea en Pxr abolió su función reguladora.
  • La eliminación de Pxr permitió el desarrollo incluso en condiciones ricas en nutrientes, evitando el desencadenante normal del hambre.
  • La mutación Pxr restauró la competencia del desarrollo a una cepa de M. xanthus cheater socialmente defectuosa.

Conclusiones:

  • Pxr actúa como un punto de control crítico que controla la transición del crecimiento al desarrollo en las mixobacterias.
  • Este estudio proporciona evidencia directa de que los reguladores de sRNA pueden impulsar la evolución adaptativa de los principales rasgos fenotípicos.
  • Las moléculas de ARNs rigen el desarrollo multicelular complejo en procariotas, facilitando la adaptación evolutiva.