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Una red global de interacción de la proteína quinasa y la fosfatasa en levaduras

Ashton Breitkreutz¹, Hyungwon Choi, Jeffrey R Sharom

¹Centre for Systems Biology, Samuel Lunenfeld Research Institute, 600 University Avenue, Toronto, Ontario, M5G 1X5, Canada.

Science (New York, N.Y.)

|May 22, 2010

Resumen

Los investigadores mapearon las interacciones de la proteína quinasa y la fosfatasa en la levadura, revelando nuevas funciones celulares. Esta red de interacción de kinasa-fosfatasa destaca las funciones en el ciclo celular, el daño al ADN y el metabolismo.

Área de la Ciencia:

Biología celular Biología celular.
La bioquímica es la bioquímica.
Biología de Sistemas Biología de Sistemas.

Sus antecedentes:

Las proteínas quinasas y las fosfatasas son reguladores cruciales de los procesos celulares.
Sus interacciones con sustratos y subunidades reguladoras son fundamentales para el control celular.

Objetivo del estudio:

Identificar y mapear la red completa de interacciones de kinasa y fosfatasa en la levadura en ciernes.
Descubrir nuevas funciones y roles reguladores de estas interacciones.

Principales métodos:

Utilizó análisis espectrométrico de masas de complejos proteicos para identificar interacciones.
Construyó una red de interacción de kinasa y fosfatasa (KPI).

Principales resultados:

Se identificaron 1844 interacciones, formando una importante red de KPI en la levadura.
Se descubrieron regiones de interacción densa que sugieren nuevos roles funcionales.
Se revelaron nuevas funciones para la Cdc14 fosfatasa en la señalización de MAPK, la respuesta al daño del ADN y el metabolismo.
Se descubrieron nuevas cinasas efectoras para el objetivo del complejo de rapamicina 1 (TORC1) en las vías metabólicas.
Encontraron una extensa red de interacciones kinasa-kinasa a través del proteoma.

Conclusiones:

La red de KPI de levadura proporciona un mapa completo de la regulación de la quinasa y la fosfatasa.
Las interacciones identificadas revelan funciones previamente desconocidas en el ciclo celular, el daño al ADN y el metabolismo.
La red de quinasa interconectada sugiere un mecanismo para coordinar las respuestas celulares.

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