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Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
Published on: April 8, 2020
Vesículas sensibles a la tensión basadas en el ensamblaje ortogonal de dos homopolímeros
Qiang Yan1, Jinying Yuan, Zhinan Cai
1Key Lab of Organic Optoelectronic & Molecular Engineering of Ministry of Education, Department of Chemistry, Tsinghua University, Beijing, 100084, China.
Journal of the American Chemical Society
|June 23, 2010
Resumen
El autoensamblaje de polímeros controlado por voltaje crea nanocápsulas sensibles. La fuerza de voltaje aplicada regula con precisión la velocidad de la formación de vesículas y la liberación de fármacos de estas estructuras supramoleculares.
Área de la Ciencia:
- Se trata de una química supramolecular.
- La ciencia de los polímeros es la ciencia de los polímeros.
- Ciencia de los materiales ciencia de los materiales.
Sus antecedentes:
- Las interacciones huésped-anfitrión son cruciales para el autoensamblaje.
- Los polímeros supramoleculares ofrecen propiedades sintonizables.
- Los sistemas de liberación controlada son vitales para la administración de fármacos.
Objetivo del estudio:
- Para desarrollar copolímeros supramoleculares de doble bloqueo sensibles a la tensión.
- Para investigar la formación y el comportamiento de las vesículas autoensambladas.
- Para controlar la cinética del ensamblaje/desensamblaje de las vesículas y la liberación de fármacos.
Principales métodos:
- Autoensamblaje ortogonal de homopolímeros decorados por el extremo (poliestireno) -beta-ciclodextrina y polióxido de etileno-ferroceno).
- Formación de vesículas supramoleculares a través de las interacciones huésped-invitado.
- Asociación / disociación reversible desencadenada por voltaje de las conexiones supramoleculares.
- Espectroscopia de luminiscencia para analizar la encapsulación y liberación de las vesículas.
Principales resultados:
- Se ha logrado el autoensamblaje ortogonal en copolímeros diblock supramoleculares (PS-beta-CD/PEO-Fc) en solución acuosa.
- Se ha demostrado el montaje y desmontaje reversible controlado por voltaje de las vesículas supramoleculares.
- Descubrió que la fuerza de la tensión aplicada dicta la velocidad de la formación y disociación de las vesículas.
- Las vesículas confirmadas funcionan como nanocápsulas con liberación molecular regulada por voltaje.
Conclusiones:
- Los copolímeros de diblock supramoleculares pueden formar vesículas sensibles al voltaje.
- La tensión aplicada ofrece un control preciso sobre la cinética del autoensamblaje y el desmontaje.
- Estas nanocápsulas reguladas por voltaje permiten perfiles de liberación de fármacos sintonizables.
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