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La activación de la autofagia durante la muerte celular requiere el receptor de engulfment Draper
Christina K McPhee1, Mary A Logan, Marc R Freeman
1Department of Cancer Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.
Nature
|June 26, 2010
Resumen
Draper (Drpr) es esencial para la autofagia durante la muerte celular programada en las glándulas salivales de Drosophila, distinguiéndola de la autofagia relacionada con la supervivencia. Este factor de engulfment se requiere dentro de las células moribundas para su auto-limpieza.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología del desarrollo Biología del desarrollo.
- Investigación de la autofagia Investigación de la autofagia.
Sus antecedentes:
- La autofagia es crucial para la supervivencia celular durante la inanición, pero su papel en la muerte celular no se entiende completamente.
- Distinguir la autofagia durante la muerte celular de la que se produce durante la supervivencia celular es un desafío clave en la biología celular.
- Las glándulas salivales de Drosophila sufren una muerte celular programada que incluye autofagia, sin envolvimiento fagocítico aparente.
Objetivo del estudio:
- Para investigar el papel de Draper (Drpr) en la autofagia durante la muerte celular programada.
- Para determinar si el Drpr distingue entre la autofagia asociada con la muerte celular y la supervivencia celular.
- Identificar los factores que regulan la autolimpieza de las células moribundas.
Principales métodos:
- Se utilizaron modelos de Drosophila melanogaster con mutaciones nulas y knockdown específico de la glándula salival del gen drpr.
- Se evaluó la degradación de las glándulas salivales y la inducción de la autofagia en mutantes drpr.
- Se examinó el efecto de la reducción drpr en la autofagia inducida por el hambre en el cuerpo graso.
- Investigó el requisito de los componentes de la vía de engulfment en la depuración de la glándula salival al morir.
Principales resultados:
- Las mutaciones nulas y el derribo de la drpr inhiben la degradación de las glándulas salivales y la inducción de la autofagia durante la muerte celular.
- El Drpr se requiere dentro de las células de las glándulas salivales moribundas para la inducción de la autofagia y la autolimpieza.
- Drpr knockdown no impidió la autofagia inducida por la inanición en el cuerpo graso, que está vinculada a la supervivencia celular.
- Los componentes de la vía de engulfment son necesarios para el aclaramiento de las glándulas salivales moribundas.
Conclusiones:
- Draper (Drpr) es esencial para la autofagia durante la muerte celular programada y la autolimpieza celular en las glándulas salivales de Drosophila.
- Drpr actúa como el primer factor identificado que diferencia la autofagia asociada con la muerte celular de la asociada con la supervivencia celular.
- Este estudio revela un papel novedoso para los factores de engulfment en la autolimpieza no fagocítica de las células moribundas.
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