Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
tRNA Activation02:26

tRNA Activation

Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...
tRNA Activation02:26

tRNA Activation

Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Kinetically Divergent Dimanganese-Tyrosyl Radical Cofactor Assembly in <i>Listeria monocytogenes</i> Class I Ribonucleotide Reductase.

Biochemistry·2026
Same author

Differentiating 5-thiooxazoles from oxazolone-coupled thioamides in RiPP natural products.

bioRxiv : the preprint server for biology·2026
Same author

Amino Acids in the RSSY Motif of Lipoyl Synthase Control Substrate Binding and Reactivity.

bioRxiv : the preprint server for biology·2026
Same author

A redox- and proton-coupled inner membrane transporter mediates copper import to the bacterial cytoplasm.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Bis-hydroxylation of Homocitrulline Catalyzed by a Multinuclear Nonheme Iron-Dependent Oxidative Enzyme during RiPP Biosynthesis.

bioRxiv : the preprint server for biology·2026
Same author

JoAnne Stubbe's Radical Path: A Story of Passion, Curiosity, and Persistence.

Annual review of biochemistry·2026

Video Experimental Relacionado

Updated: Jun 10, 2026

NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases
10:24

NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases

Published on: June 30, 2019

Bases estructurales para la activación de la reductasa ribonucleótida de clase Ib.

Amie K Boal1, Joseph A Cotruvo, JoAnne Stubbe

  • 1Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208, USA.

Science (New York, N.Y.)
|August 7, 2010
PubMed
Resumen

La ribonucleotida reductasa de clase Ib en E. coli utiliza cofactores de manganeso o hierro. Los estudios estructurales revelan distintas vías de activación y un canal clave para el ensamblaje del cofactor de manganeso, que involucra a la proteína NrdI.

Más Videos Relacionados

High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis
09:04

Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis

Published on: July 26, 2018

Videos de Experimentos Relacionados

Last Updated: Jun 10, 2026

NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases
10:24

NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases

Published on: June 30, 2019

High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis
09:04

Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis

Published on: July 26, 2018

Área de la Ciencia:

  • La bioquímica es la bioquímica.
  • Biología Estructural Biología estructural.
  • Enzimología Enzimología.

Sus antecedentes:

  • La ribonucleotida reductasa (RNR) de clase Ib de Escherichia coli utiliza un radical tirosílico (Y•) de Mn(III) 2-o un cofactor Fe(III) 2-Y• para la reducción de nucleótidos.
  • El ensamblaje del cofactor de hierro es espontáneo con O2, pero la activación del cofactor de manganeso requiere la flavoproteína reducida NrdI.

Objetivo del estudio:

  • Para aclarar los mecanismos estructurales subyacentes a la activación del cofactor en la clase Ib RNR.
  • Para investigar el papel de NrdI en el ensamblaje del cofactor manganeso.

Principales métodos:

  • Cristalografía de rayos X de E. coli Mn(II) 2-NrdF y Fe(II) 2-NrdF.
  • Análisis estructural de Mn(II) 2-NrdF en complejo con NrdI.II. reducido y oxidado.
  • Detección cristalográfica de los intermediarios de reacción.

Principales resultados:

  • Se observaron entornos de coordinación distintos para Mn{II}2-NrdF y Fe{II}2-NrdF, lo que sugiere diferentes sitios de unión de oxidantes.
  • Se identificó un canal continuo que conecta el cofactor de flavina NrdI con el sitio activo Mn(II) 2 en Mn(II) 2-NrdF.
  • Se detectó un presunto intermediario de peróxido dentro de este canal, apoyando el mecanismo catalítico propuesto.

Conclusiones:

  • Los hallazgos revelan distintas vías estructurales para la activación de los cofactores de manganeso y hierro en la clase Ib RNR.
  • El ensamblaje de cofactor de manganeso mediado por NrdI implica un canal directo que facilita la transferencia de oxidantes y la formación de intermedios.