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MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

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Updated: Jun 10, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

Los microARN de mamíferos actúan predominantemente para disminuir los niveles de ARNm objetivo.

Huili Guo1, Nicholas T Ingolia, Jonathan S Weissman

  • 1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.

Nature
|August 13, 2010
PubMed
Resumen
Este resumen es generado por máquina.

Los microARN (miRNA) reducen principalmente la producción de proteínas al disminuir los niveles de ARN mensajero objetivo (ARNm), no al inhibir la traducción. Este hallazgo destaca la desestabilización del ARNm como el principal mecanismo para la regulación génica mediada por el ARNm.

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Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

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Last Updated: Jun 10, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
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Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • Genética La genética.
  • Regulación genética Reglamento genético.

Sus antecedentes:

  • Los microARN (miRNA) son pequeños ARN no codificantes que regulan la expresión génica.
  • La represión génica mediada por miRNA puede ocurrir a través de la inhibición traslacional o la degradación del mRNA.
  • La contribución relativa de estos mecanismos, especialmente para los objetivos endógenos, sigue sin caracterizarse en gran medida.

Objetivo del estudio:

  • Cuantificar las contribuciones de la represión traslacional y la degradación del ARNm a la reducción de la producción de proteínas mediada por miARN.
  • Para comparar estos efectos tanto para objetivos de miRNA ectópicos como endógenos.
  • Para dilucidar el mecanismo predominante por el cual los miRNA regulan la producción de proteínas.

Principales métodos:

  • Utilizó perfiles de ribosoma para medir las tasas globales de síntesis de proteínas.
  • Se evaluaron simultáneamente los cambios en los niveles de ARN mensajero (ARNm).
  • Se analizaron tanto los objetivos de miRNA expresados ectópicamente como los regulados endógenamente.

Principales resultados:

  • Los niveles reducidos de ARNm representaron la mayoría (> = 84%) de la disminución de la producción de proteínas tanto para las interacciones de ARNm ectópicas como endógenas.
  • El impacto de la regulación de miRNA en la producción de proteínas está estrechamente correlacionado con los cambios en la abundancia de mRNA.
  • La desestabilización del ARNm se identificó como el principal impulsor de la reducción de proteínas inducida por el ARNm.

Conclusiones:

  • El silenciamiento genético mediado por miRNA se produce predominantemente a través de la desestabilización de los ARNm diana.
  • Los cambios en los niveles de ARNm sirven como un indicador confiable del impacto regulatorio del ARNm.
  • Comprender este mecanismo es crucial para comprender la regulación génica y desarrollar terapias basadas en miRNA.