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Un surco de hendidura independiente del husillo posiciona el camino de posicionamiento
Clemens Cabernard1, Kenneth E Prehoda, Chris Q Doe
1Howard Hughes Medical Institute, University of Oregon, Eugene, Oregon 97403, USA.
Nature
|September 3, 2010
Resumen
Un nuevo mecanismo independiente del huso posiciona el surco de escisión en los neuroblastos de Drosophila. La vía de polaridad cortical de A Pins dirige las proteínas de surco a la corteza basal, lo que influye en la determinación del sitio de división celular.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología del desarrollo Biología del desarrollo.
- Genética La genética.
Sus antecedentes:
- El huso mitótico se considera tradicionalmente el principal determinante del sitio del surco de escisión durante la división celular de los metazoos.
- La existencia de mecanismos alternativos para el posicionamiento de surcos de escisión sigue siendo en gran medida inexplorada.
Objetivo del estudio:
- Investigar posibles mecanismos independientes del husillo que gobiernan la localización del surco de escisión en los neuroblastos de Drosophila.
- Para dilucidar el papel de las vías de polaridad cortical en la regulación de la determinación del sitio de división celular.
Principales métodos:
- Utilizó los neuroblastos de Drosophila como un sistema modelo.
- Investigó la localización de las proteínas clave del surco (Pavarotti, Anillin, Myosin) en la corteza basal.
- Se examinaron los efectos de la rotación y el desplazamiento del husillo en la formación de surcos.
- Analizó la participación de la vía de polaridad cortical de Pins.
Principales resultados:
- Se identificó un mecanismo independiente del husillo para el posicionamiento del surco de escisión en los neuroblastos de Drosophila.
- Se demostró que las proteínas de la vía de polaridad cortical de Pins localizan las proteínas de surco temprano y tardío en la corteza basal de los neuroblastos.
- Se demostró que esta vía puede inducir un surco basalmente desplazado incluso sin un huso mitótico.
- Observó la formación de dos surcos distintos (inducidos por la polaridad y inducidos por el huso) al manipular el huso.
Conclusiones:
- Un mecanismo independiente del husillo, mediado por la vía de polaridad cortical de Pins, contribuye al posicionamiento del surco de escisión en los neuroblastos de Drosophila.
- Este mecanismo puede ser crucial para la división celular en otras células altamente polarizadas, incluidos los progenitores neurales de mamíferos.
- Los hallazgos desafían el papel exclusivo del huso mitótico en la determinación del sitio de división celular.
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