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Lateral Root Inducible System in Arabidopsis and Maize
Published on: January 14, 2016
La expresión génica oscilante determina la competencia para la ramificación periódica de las raíces de Arabidopsis
Miguel A Moreno-Risueno1, Jaimie M Van Norman, Antonio Moreno
1Department of Biology and Institute for Genome Sciences and Policy Center for Systems Biology, Duke University, Durham, NC 27708, USA.
Science (New York, N.Y.)
|September 11, 2010
Resumen
Las raíces de las plantas desarrollan raíces laterales periódicamente, guiadas por pulsos cíclicos de expresión génica que establecen sitios de pre-ramas. Este mecanismo periódico en las plantas comparte similitudes con los relojes moleculares que se encuentran en los animales.
Área de la Ciencia:
- Biología del desarrollo vegetal Biología del desarrollo vegetal
- Genética molecular genética molecular.
- Desarrollo de las raíces.
Sus antecedentes:
- Las plantas y los animales exhiben una producción periódica de unidades de desarrollo modulares.
- La formación de raíces laterales en las plantas ocurre periódicamente a lo largo del eje de la raíz primaria, pero el mecanismo subyacente sigue sin estar claro.
Objetivo del estudio:
- Investigar el mecanismo molecular que regula la formación periódica de raíces laterales en las plantas.
- Para identificar genes y procesos regulatorios involucrados en el establecimiento de sitios de pre-ramas y la periodicidad de la raíz.
Principales métodos:
- Utilizó un gen reportero para visualizar pulsos de expresión cíclica en futuros sitios de raíz lateral.
- Realizó ensayos de microarray y promotor-luciferasa para identificar genes oscilantes en la punta de la raíz.
- Realizó estudios genéticos para evaluar el papel de los reguladores transcripcionales en la periodicidad del desarrollo.
Principales resultados:
- Los pulsos de expresión cíclica de un gen reportero marcan los sitios de pre-ramas, que preceden a la formación de raíces laterales.
- Tanto la producción en el sitio de la pre-rama como la flexión de la raíz exhiben periodicidad.
- Identificó dos conjuntos de genes que oscilan en fases opuestas en la punta de la raíz.
- Descubrió que los reguladores transcripcionales oscilantes específicos son esenciales para la periodicidad de los procesos de desarrollo.
Conclusiones:
- Un mecanismo molecular que implica la expresión génica oscilante regula el desarrollo periódico de la raíz lateral en las plantas.
- Este mecanismo establece los sitios de pre-rama e influye en la periodicidad de flexión de la raíz.
- Las características de reloj molecular identificadas sugieren principios conservados en la periodicidad del desarrollo a través de los reinos.
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